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咖啡因和透明质酸对人皮肤成纤维细胞胶原蛋白生物合成的影响。

Influence of caffeine and hyaluronic acid on collagen biosynthesis in human skin fibroblasts.

作者信息

Donejko Magdalena, Przylipiak Andrzej, Rysiak Edyta, Głuszuk Katarzyna, Surażyński Arkadiusz

机构信息

Department of Esthetic Medicine, Faculty of Pharmacy, Medical University of Białystok, Białystok, Poland.

Department of Medicinal Chemistry, Faculty of Pharmacy, Medical University of Białystok, Białystok, Poland.

出版信息

Drug Des Devel Ther. 2014 Oct 15;8:1923-8. doi: 10.2147/DDDT.S69791. eCollection 2014.

DOI:10.2147/DDDT.S69791
PMID:25342885
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4206198/
Abstract

AIM

The aim of this study was to evaluate the effect of caffeine on collagen biosynthesis in human skin fibroblasts and the influence of hyaluronic acid (HA) on this process.

MATERIALS AND METHODS

Collagen, [(3)H]-thymidine incorporation, and prolidase activity were measured in confluent human skin fibroblast cultures that had been treated with 1, 2, and 5 mM caffeine and with caffeine and 500 μg/mL HA. Western immunoblot analysis was performed to evaluate expression of β1-integrin receptor, insulin-like growth factor receptor phospho-Akt protein and mitogen-activated protein kinase (phospho-extracellular signal-regulated kinase).

RESULTS

Caffeine inhibited collagen biosynthesis in a dose-dependent manner. The mechanism of this process was found at the level of prolidase activity. Caffeine significantly inhibited the enzyme activity. The addition of HA had no effect on collagen biosynthesis or prolidase activity in fibroblasts incubated with caffeine. Caffeine also had an inhibitory effect on DNA biosynthesis. HA, however, did not have any significant effect on this process. The inhibition of the expression of β1-integrin and insulin-like growth factor receptor in fibroblasts incubated with the caffeine indicates a possible mechanism of inhibition of collagen biosynthesis.

CONCLUSION

Caffeine reduces collagen synthesis in human cultured skin fibroblasts. HA did not have any significant protective effect on this process. This is the first study to our knowledge that reports caffeine-induced inhibition of collagen synthesis in human skin fibroblasts.

摘要

目的

本研究旨在评估咖啡因对人皮肤成纤维细胞中胶原蛋白生物合成的影响以及透明质酸（HA）对该过程的影响。

材料与方法

在汇合的人皮肤成纤维细胞培养物中测量胶原蛋白、[³H] - 胸腺嘧啶核苷掺入量和脯氨酰肽酶活性，这些培养物分别用1、2和5 mM咖啡因以及咖啡因与500 μg/mL HA处理。进行蛋白质免疫印迹分析以评估β1整合素受体、胰岛素样生长因子受体磷酸化Akt蛋白和丝裂原活化蛋白激酶（磷酸化细胞外信号调节激酶）的表达。

结果

咖啡因以剂量依赖方式抑制胶原蛋白生物合成。该过程的机制在脯氨酰肽酶活性水平被发现。咖啡因显著抑制该酶活性。在与咖啡因一起孵育的成纤维细胞中添加HA对胶原蛋白生物合成或脯氨酰肽酶活性没有影响。咖啡因对DNA生物合成也有抑制作用。然而，HA对该过程没有任何显著影响。在与咖啡因一起孵育的成纤维细胞中β1整合素和胰岛素样生长因子受体表达的抑制表明了抑制胶原蛋白生物合成的一种可能机制。

结论

咖啡因减少人培养皮肤成纤维细胞中的胶原蛋白合成。HA对该过程没有任何显著的保护作用。据我们所知，这是第一项报道咖啡因诱导人皮肤成纤维细胞中胶原蛋白合成受抑制的研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5942/4206198/d8ce63aacec8/dddt-8-1923Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5942/4206198/9d7d088b14cb/dddt-8-1923Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5942/4206198/6087a4b56b02/dddt-8-1923Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5942/4206198/16091ef07799/dddt-8-1923Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5942/4206198/d8ce63aacec8/dddt-8-1923Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5942/4206198/9d7d088b14cb/dddt-8-1923Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5942/4206198/6087a4b56b02/dddt-8-1923Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5942/4206198/16091ef07799/dddt-8-1923Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5942/4206198/d8ce63aacec8/dddt-8-1923Fig4.jpg

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