a Department of Oncology-Pathology , Karolinska Institutet , Stockholm , Sweden.
b Department of Medicine Solna , Karolinska Institutet , Stockholm , Sweden.
Infect Dis (Lond). 2017 Aug;49(8):588-593. doi: 10.1080/23744235.2017.1300319. Epub 2017 Mar 15.
The role of human papillomavirus (HPV) in tonsillar squamous cell carcinomas (TSCC) is of interest, since a considerable proportion of TSCC in Sweden and other Western countries is HPV positive. Nevertheless, the natural history of HPV in normal tonsils, and the progression from localized infection to pre-malignant lesion to cancer are poorly understood. The aim of this study was to investigate whether HPV types found in mouthwash samples correlated to those in tonsillar tissue from the same individuals undergoing tonsillectomy.
Mouthwash samples from 232 patients, aged 3-56 years, undergoing tonsillectomy, the majority with chronic tonsillitis, were collected at the time of surgery and analysed for the presence of 27 HPV types by a bead based multiplex assay.
An HPV prevalence of 10.3% (24/232) was observed in mouthwash samples, with HPV 69 being the dominant type (10/24). Ten patients were positive for high risk HPV (HPV 16, 33, 35, 45, 56, 59). None of the tonsils resected from patients with HPV-positive mouthwash samples were positive for HPV.
Despite an oral HPV prevalence of 10.3% in mouthwash samples from tonsillectomized patients, with dominance of HPV 69, none of the corresponding tonsillar samples exhibited the presence of HPV.
人乳头瘤病毒（HPV）在扁桃体鳞状细胞癌（TSCC）中的作用引起了关注，因为在瑞典和其他西方国家相当一部分 TSCC 是 HPV 阳性的。然而，HPV 在正常扁桃体中的自然史，以及从局部感染到癌前病变再到癌症的进展过程仍知之甚少。本研究旨在探讨从接受扁桃体切除术的同一患者的扁桃体组织中发现的 HPV 类型是否与在漱口水中样本中发现的 HPV 类型相关。
在扁桃体切除术时，收集了 232 名年龄在 3-56 岁的患者的漱口口水样本，其中大多数患有慢性扁桃体炎，并通过基于珠的多重分析来检测 27 种 HPV 类型的存在情况。
在漱口水中样本中观察到 HPV 的流行率为 10.3%（24/232），HPV 69 是主要类型（10/24）。10 名患者的高危 HPV（HPV 16、33、35、45、56、59）呈阳性。从 HPV 阳性漱口水中样本的扁桃体切除患者中，没有一个扁桃体样本呈 HPV 阳性。
尽管在接受扁桃体切除术的患者的漱口水中样本中 HPV 的流行率为 10.3%，以 HPV 69 为主导，但在相应的扁桃体样本中均未发现 HPV 的存在。
