Mujtaba Hasan, Wang Ying, Duan Yixin, Cao Meng, Zhang Nana, Batool Iffat, Murtaza Ali, Chen Xiaoli, Wang Yili
Institute for Cancer Research, School of Basic Medical Science, Xi'an Jiaotong University, Xian, Shaanxi, 710061, China.
Department of Pediatrics, Fauji Foundation Hospital, Rawalpindi, Pakistan.
Pathol Res Pract. 2018 Oct;214(10):1713-1718. doi: 10.1016/j.prp.2018.08.032. Epub 2018 Sep 6.
The expected corresponding increase in tonsillar human papillomavirus (HPV) infection associated with increasing incidence of oropharyngeal squamous cell carcinoma (OPSCC) substantiate the evaluation of normal tonsillar tissue in different population. The epidemiology of HPV in tonsillar tissue varies geographically. This study evaluated samples from two countries to determine the prevalence in respective samples.
To characterize HPV infection in non-malignant tonsillar tissue from Shaanxi, China (herein after referred to as China) and Punjab, Pakistan (herein after referred to as Pakistan).
The DNA extracted from formalin-fixed, paraffin-embedded tumor free tonsillar tissue was evaluated using polymerase chain reaction (PCR). A total of 367 cases from China and 139 cases from Pakistan were screened for HPV DNA using GP5+/GP6+ consensus primer. Genotype of the positive cases was determined for common HPV types (6, 11, 16, 18, 52, 58) simultaneously by type-specific PCR.
The mean age of cohorts in China was 13.42 (Median age 7, Range 2-72 years) while in Pakistan it was 10.77 (Median age 8, Range 3-42 years) the gender distribution was 61.6% male in China and in Pakistan they were 56.8%, rest were females. The overall prevalence of HPV in China was 2.45% and 2.16% in Pakistan. High risk human papillomavirus was 1.63% in China with 5 cases positive for HPV 16 and HPV 58 in 1 case. In Pakistan, 2 cases (1.43%) of HPV 16 were detected. Low-risk types include HPV 11 present in 2 cases from China, while HPV 6 was detected in 1 case each from both the countries.
A low prevalence of HPV was found in China and Pakistan; high-risk and low-risk HPV were detectable in tonsillar tissue from both countries. Age stratification (< 5 years, 5-14 years, 15-25 years, > 25 years) suggest that sexual and non-sexual transmission of the virus can occur. The difference in the genotype distribution geographically within China and with Pakistan was observed in the case of HPV 58. The most common type in both the countries was HPV 16.
口咽鳞状细胞癌（OPSCC）发病率上升，与之相应的扁桃体人乳头瘤病毒（HPV）感染预期增加，这证实了对不同人群正常扁桃体组织进行评估的必要性。扁桃体组织中HPV的流行病学在地理上存在差异。本研究评估了来自两个国家的样本，以确定各自样本中的患病率。
描述中国陕西（以下简称中国）和巴基斯坦旁遮普省（以下简称巴基斯坦）非恶性扁桃体组织中的HPV感染情况。
使用聚合酶链反应（PCR）评估从福尔马林固定、石蜡包埋的无肿瘤扁桃体组织中提取的DNA。使用GP5+/GP6+通用引物对来自中国的367例样本和来自巴基斯坦的139例样本进行HPV DNA筛查。通过型特异性PCR同时确定阳性病例中常见HPV类型（6、11、16、18、52、58）的基因型。
中国队列的平均年龄为13.42岁（中位年龄7岁，范围2 - 72岁），而巴基斯坦为10.77岁（中位年龄8岁，范围3 - 42岁）。中国的性别分布为男性占61.6%，巴基斯坦为56.8%，其余为女性。中国HPV的总体患病率为2.45%，巴基斯坦为2.16%。中国高危型人乳头瘤病毒为1.63%，其中5例HPV 16阳性，1例HPV 58阳性。在巴基斯坦，检测到2例（1.43%）HPV 16。低危型包括中国2例样本中的HPV 11，而两个国家各有1例样本检测到HPV 6。
在中国和巴基斯坦发现HPV患病率较低；在两国的扁桃体组织中均可检测到高危型和低危型HPV。年龄分层（<5岁、5 - 14岁、15 - 25岁、>25岁）表明该病毒可通过性传播和非性传播。在中国国内以及与巴基斯坦相比，HPV 58的基因型分布在地理上存在差异。两国最常见的类型均为HPV 16。
