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Rapid detection of viral-specific antibodies by enzyme-linked immunosorbent assay (ELISA).通过酶联免疫吸附测定（ELISA）快速检测病毒特异性抗体。
Vet Immunol Immunopathol. 1987 Dec;17(1-4):453-64. doi: 10.1016/0165-2427(87)90161-9.
2
Competitive enzyme immunoassays for the rapid detection of antibodies to feline infectious peritonitis virus polypeptides.用于快速检测猫传染性腹膜炎病毒多肽抗体的竞争性酶免疫测定法。
J Clin Microbiol. 1985 Sep;22(3):395-401. doi: 10.1128/jcm.22.3.395-401.1985.
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The use of enzyme-linked immunosorbent assay systems for serology and antigen detection in parvovirus, coronavirus and rotavirus infections in dogs in The Netherlands.荷兰用于犬细小病毒、冠状病毒和轮状病毒感染血清学及抗原检测的酶联免疫吸附测定系统的应用
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Animal virus infections that defy vaccination: equine infectious anemia, caprine arthritis-encephalitis, maedi-visna, and feline infectious peritonitis.难以通过疫苗接种预防的动物病毒感染：马传染性贫血、山羊关节炎-脑炎、梅迪-维斯纳病和猫传染性腹膜炎。
Adv Vet Sci Comp Med. 1989;33:413-28. doi: 10.1016/b978-0-12-039233-9.50017-2.
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Feline infectious peritonitis virus antibody test using enzyme-linked immunosorbent assay.使用酶联免疫吸附测定法进行猫传染性腹膜炎病毒抗体检测。
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Lab Anim Sci. 1984 Dec;34(6):592-7.
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Micro-neutralization test with canine coronavirus for detection of coronavirus antibodies in dogs and cats.用犬冠状病毒进行微中和试验以检测犬猫体内的冠状病毒抗体。
Nihon Juigaku Zasshi. 1987 Jun;49(3):563-5. doi: 10.1292/jvms1939.49.563.

引用本文的文献

1
Detection of equine infectious anemia viral RNA in plasma samples from recently infected and long-term inapparent carrier animals by PCR.通过聚合酶链反应（PCR）检测近期感染和长期隐性携带动物血浆样本中的马传染性贫血病毒RNA。
J Clin Microbiol. 1996 Jun;34(6):1481-7. doi: 10.1128/jcm.34.6.1481-1487.1996.
2
Isolation of peplomer glycoprotein E2 of transmissible gastroenteritis virus and application in enzyme-linked immunosorbent assay.传染性胃肠炎病毒纤突糖蛋白E2的分离及其在酶联免疫吸附测定中的应用。
J Clin Microbiol. 1989 Aug;27(8):1754-8. doi: 10.1128/jcm.27.8.1754-1758.1989.

本文引用的文献

1
Maternally derived immunity to canine parvovirus infection: transfer, decline, and interference with vaccination.母源抗犬细小病毒感染的免疫力：传递、衰退及对疫苗接种的干扰
J Am Vet Med Assoc. 1982 Jan 1;180(1):37-42.
2
Canine parvovirus serum neutralizing antibody assay: assessment of factors responsible for disparity of results between tests.犬细小病毒血清中和抗体检测：对检测结果差异相关因素的评估
Cornell Vet. 1983 Jan;73(1):52-7.
3
An enteric coronavirus infection of cats and its relationship to feline infectious peritonitis.猫的一种肠道冠状病毒感染及其与猫传染性腹膜炎的关系。
Am J Vet Res. 1981 Mar;42(3):368-77.
4
Antigenic relationships among homologous structural polypeptides of porcine, feline, and canine coronaviruses.猪、猫和犬冠状病毒同源结构多肽之间的抗原关系。
Infect Immun. 1982 Sep;37(3):1148-55. doi: 10.1128/iai.37.3.1148-1155.1982.
5
Isolation and comparative biochemical properties of the major internal polypeptides of equine infectious anemia virus.马传染性贫血病毒主要内部多肽的分离及比较生化特性
J Virol. 1982 Jun;42(3):1029-38. doi: 10.1128/JVI.42.3.1029-1038.1982.
6
Competitive enzyme immunoassays for the rapid detection of antibodies to feline infectious peritonitis virus polypeptides.用于快速检测猫传染性腹膜炎病毒多肽抗体的竞争性酶免疫测定法。
J Clin Microbiol. 1985 Sep;22(3):395-401. doi: 10.1128/jcm.22.3.395-401.1985.
7
Rapid enzyme-linked immunosorbent assay for detecting antibodies to canine parvovirus.用于检测犬细小病毒抗体的快速酶联免疫吸附测定
Am J Vet Res. 1985 Apr;46(4):859-63.
8
Comparison of diagnostic tests for the detection of equine infectious anemia antibody.
J Vet Diagn Invest. 1989 Jan;1(1):50-2. doi: 10.1177/104063878900100114.
9
Equine infectious anemia: current knowledge.
J Am Vet Med Assoc. 1979 Apr 1;174(7):727-33.

通过酶联免疫吸附测定（ELISA）快速检测病毒特异性抗体。

Rapid detection of viral-specific antibodies by enzyme-linked immunosorbent assay (ELISA).

作者信息

Winston S, Fiscus S, Hesterberg L, Matsushita T, Mildbrand M, Porter J, Teramoto Y

机构信息

Allelix Inc., Mississauga, Ontario.

出版信息

Vet Immunol Immunopathol. 1987 Dec;17(1-4):453-64. doi: 10.1016/0165-2427(87)90161-9.

DOI:10.1016/0165-2427(87)90161-9
PMID:2829416
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7133761/
Abstract

The development of three separate rapid ELISAs for detecting antibodies in host serum to three different viruses is described. These include: 1. A direct antigen assay using enzyme labelled anti-canine Ig for detecting antibodies to canine parvovirus, 2. A competitive ELISA using a feline infectious peritonitis virus-specific monoclonal antibody labelled with enzyme, and 3. A competitive ELISA using an equine infectious anemia virus-specific monoclonal antibody and enzyme labelled antigen, p. 26. The utility and benefits of each of the three approaches is emphasized.

摘要

本文描述了三种用于检测宿主血清中针对三种不同病毒抗体的独立快速酶联免疫吸附测定（ELISA）的开发情况。这些方法包括：1. 使用酶标记抗犬免疫球蛋白的直接抗原测定法，用于检测抗犬细小病毒抗体；2. 使用酶标记的猫传染性腹膜炎病毒特异性单克隆抗体的竞争性ELISA；3. 使用马传染性贫血病毒特异性单克隆抗体和酶标记抗原的竞争性ELISA，第26页。文中强调了这三种方法各自的实用性和优势。