通过酶联免疫吸附测定（ELISA）快速检测病毒特异性抗体。
Rapid detection of viral-specific antibodies by enzyme-linked immunosorbent assay (ELISA).
作者信息
Winston S, Fiscus S, Hesterberg L, Matsushita T, Mildbrand M, Porter J, Teramoto Y
机构信息
Allelix Inc., Mississauga, Ontario.
出版信息
Vet Immunol Immunopathol. 1987 Dec;17(1-4):453-64. doi: 10.1016/0165-2427(87)90161-9.
Abstract
The development of three separate rapid ELISAs for detecting antibodies in host serum to three different viruses is described. These include: 1. A direct antigen assay using enzyme labelled anti-canine Ig for detecting antibodies to canine parvovirus, 2. A competitive ELISA using a feline infectious peritonitis virus-specific monoclonal antibody labelled with enzyme, and 3. A competitive ELISA using an equine infectious anemia virus-specific monoclonal antibody and enzyme labelled antigen, p. 26. The utility and benefits of each of the three approaches is emphasized.
摘要
本文描述了三种用于检测宿主血清中针对三种不同病毒抗体的独立快速酶联免疫吸附测定（ELISA）的开发情况。这些方法包括：1. 使用酶标记抗犬免疫球蛋白的直接抗原测定法，用于检测抗犬细小病毒抗体；2. 使用酶标记的猫传染性腹膜炎病毒特异性单克隆抗体的竞争性ELISA；3. 使用马传染性贫血病毒特异性单克隆抗体和酶标记抗原的竞争性ELISA，第26页。文中强调了这三种方法各自的实用性和优势。
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本文引用的文献
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J Am Vet Med Assoc. 1982 Jan 1;180(1):37-42.
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An enteric coronavirus infection of cats and its relationship to feline infectious peritonitis.猫的一种肠道冠状病毒感染及其与猫传染性腹膜炎的关系。
Am J Vet Res. 1981 Mar;42(3):368-77.
4
Antigenic relationships among homologous structural polypeptides of porcine, feline, and canine coronaviruses.猪、猫和犬冠状病毒同源结构多肽之间的抗原关系。
Infect Immun. 1982 Sep;37(3):1148-55. doi: 10.1128/iai.37.3.1148-1155.1982.
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J Virol. 1982 Jun;42(3):1029-38. doi: 10.1128/JVI.42.3.1029-1038.1982.
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