Mochizuki M, Furukawa H
Department of Veterinary Medicine, Faculty of Agriculture, Kagoshima University, Japan.
Comp Immunol Microbiol Infect Dis. 1989;12(4):139-46. doi: 10.1016/0147-9571(89)90062-3.
From the reasons that canine coronavirus (CCV) grows more efficiently than feline coronavirus in a cell culture and they are mutually related in their antigenicities, an enzyme-linked immunosorbent assay (ELISA) using CCV-infected feline kidney (CRFK) cells as substrate antigens was developed for detection of anti-coronavirus antibodies in cats. It was indispensable for generating coronavirus-specific ELISA antibody activities that the sample was applied to the mock-infected, normal CRFK cells in parallel with the CCV-infected cells and then the optical density values given by the mock-infected cell antigen were subtracted from those given by the virus-infected cell antigen. On the basis of ELISA antibody titers obtained in sera from the cats experimentally infected with CCV and from the spontaneous feline infectious peritonitis (FIP) cases, the ELISA described in the present study was found to be applicable as a simple and easy serologic test which was able to detect anti-coronavirus antibodies as efficiently as the indirect immunofluorescence assay with homologous FIP virus.
由于犬冠状病毒（CCV）在细胞培养中比猫冠状病毒生长得更高效，且它们在抗原性上相互关联，因此开发了一种以感染CCV的猫肾（CRFK）细胞为底物抗原的酶联免疫吸附测定（ELISA），用于检测猫体内的抗冠状病毒抗体。将样品同时应用于模拟感染的正常CRFK细胞和感染CCV的细胞，然后从病毒感染细胞抗原给出的光密度值中减去模拟感染细胞抗原给出的光密度值，这对于产生冠状病毒特异性ELISA抗体活性是必不可少的。根据实验感染CCV的猫血清和自发性猫传染性腹膜炎（FIP）病例血清中获得的ELISA抗体滴度，本研究中描述的ELISA被发现可作为一种简单易行的血清学检测方法，能够像使用同源FIP病毒的间接免疫荧光测定一样有效地检测抗冠状病毒抗体。
