Li Xinyu, Sun Dandan, Li Xu, Zhu Dashuai, Jia Zhenzhen, Jiao Jiajia, Wang Kai, Kong Deling, Zhao Xin, Xu Lin, Zhao Qiang, Chen Dongyan, Feng Xizeng
State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China.
The Institute of Robotics and Automatic Information Systems, Tianjin Key Laboratory of Intelligent Robotics, Nankai University, Tianjin 300071, China.
Biomater Sci. 2017 Mar 28;5(4):849-859. doi: 10.1039/c7bm00068e.
Carbon nanomaterials have been used to treat neurodegenerative diseases and neural disorders due to their diverse molecular structures. Corannulene is a three-dimensional π-bowl carbon nanomaterial that is different from planar PAHs, fullerenes and carbon nanotubes, but little is known about its biological functions. Herein, corannulene was functionalized with mPEG-DESP to prepare PEGylation corannulene nanoparticles (PEGylation CoNps). The synthesized PEGylation CoNps shows enhanced solubility and reduced aggregation when compared corannulene. Then, in vivo experiments were performed to determine the effects of PEGylation CoNps on the neural system. We found that PEGylation CoNps treatment increased short resting bouts, decreased locomotion activities and enhanced the response to stress. Most of these behavioral changes suggest that PEGylation CoNps lead to a greater reflection to stress, which is associated with neurotransmitter expression and neurogenesis. In line with the hypothesis, we found that PEGylation CoNps administration enhanced TH, DCX and MAP-2 expression in the hippocampus. These results indicated that PEGylation CoNps enhanced the neurogenesis of mice. Furthermore, pathological analysis showed that PEGylation CoNps caused little inflammation. These findings suggest that PEGylation CoNps are a potential functionalized carbon nanomaterial for promoting neurogenesis.
由于其多样的分子结构，碳纳米材料已被用于治疗神经退行性疾病和神经紊乱。碗烯是一种三维π碗状碳纳米材料，与平面多环芳烃、富勒烯和碳纳米管不同，但其生物学功能鲜为人知。在此，碗烯用甲氧基聚乙二醇-二硬脂酰磷脂酰乙醇胺（mPEG-DESP）进行功能化处理，以制备聚乙二醇化碗烯纳米颗粒（PEGylation CoNps）。与碗烯相比，合成的PEGylation CoNps显示出增强的溶解性和减少的聚集。然后，进行体内实验以确定PEGylation CoNps对神经系统的影响。我们发现，PEGylation CoNps处理增加了短时间的静息发作，降低了运动活性，并增强了对应激的反应。这些行为变化大多表明，PEGylation CoNps导致对应激有更大的反应，这与神经递质表达和神经发生有关。与该假设一致，我们发现给予PEGylation CoNps可增强海马体中酪氨酸羟化酶（TH）、双皮质素（DCX）和微管相关蛋白2（MAP-2）的表达。这些结果表明，PEGylation CoNps增强了小鼠的神经发生。此外，病理分析表明，PEGylation CoNps引起的炎症很小。这些发现表明，PEGylation CoNps是一种潜在的用于促进神经发生的功能化碳纳米材料。
