高分辨率表型分析天然产物对单细胞水平的影响。
High-resolution phenotypic profiling of natural products-induced effects on the single-cell level.
机构信息
Red Sea Research Center, Division of Biological and Environmental Science and Engineering (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia.
出版信息
Sci Rep. 2017 Mar 15;7:44472. doi: 10.1038/srep44472.
Abstract
Natural products (NPs) are highly evolved molecules making them a valuable resource for new therapeutics. Here we demonstrate the usefulness of broad-spectrum phenotypic profiling of NP-induced perturbations on single cells with imaging-based High-Content Screening to inform on physiology, mechanisms-of-actions, and multi-level toxicity. Our technology platform aims at broad applicability using a comprehensive marker panel with standardized settings streamlined towards an easy implementation in laboratories dedicated to natural products research.
摘要
天然产物 (NPs) 是高度进化的分子，使它们成为新疗法的宝贵资源。在这里，我们展示了基于成像的高通量筛选对 NP 诱导的单细胞扰动进行广谱表型分析在生理学、作用机制和多层次毒性方面的有用性。我们的技术平台旨在通过使用全面的标记面板和标准化设置实现广泛的适用性，这些设置简化了专门从事天然产物研究的实验室的实施。
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