Sakamoto Y, Sakai M, Watari T
Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, Japan.
J Vet Intern Med. 2017 May;31(3):764-769. doi: 10.1111/jvim.14687. Epub 2017 Mar 14.
Endothelin (ET)-1 is a 21-amino-acid peptide with potent vasoactive properties, which increases intrahepatic resistance in patients with chronic hepatitis (CH) or cirrhosis. ET-1 concentrations have not been investigated in dogs with CH.
HYPOTHESIS/OBJECTIVES: This study compared hepatic and plasma ET-1 levels in healthy dogs and in dogs with CH, and examined the relationship between the plasma ET-1 level and portal vein pressure in dogs with CH.
Fourteen healthy dogs and twenty dogs with CH were used in this study.
Prospective case-control study. Hepatic ET-1 mRNA expression was determined by real-time reverse transcription polymerase chain reaction, and hepatic and plasma ET-1 levels were assessed using ELISA. Splenic pulp pressure (SPP), as an indicator of portal vein pressure, was measured laparoscopically.
Hepatic ET-1 mRNA levels were 3.7 times higher in dogs with CH than in healthy dogs (P = .008). The median hepatic and plasma ET-1 protein levels were significantly higher in dogs with CH than in healthy dogs (13.20 pg/mg wet liver vs. 3.42 pg/mg wet liver, P = .004, and 0.99 pg/mL vs. 0.71 pg/mL, P = .013, respectively). Moreover, there was a weak but significant correlation between plasma ET-1 level and SPP in dogs with CH (P = .036; r = 0.53).
The results indicate that ET-1 might play an important role in the pathogenesis of portal hypertension caused by CH.
内皮素（ET）-1是一种具有强大血管活性特性的21个氨基酸的肽，可增加慢性肝炎（CH）或肝硬化患者的肝内阻力。尚未对患CH的犬的ET-1浓度进行研究。
假设/目的：本研究比较了健康犬和患CH的犬的肝脏和血浆ET-1水平，并研究了患CH的犬的血浆ET-1水平与门静脉压力之间的关系。
本研究使用了14只健康犬和20只患CH的犬。
前瞻性病例对照研究。通过实时逆转录聚合酶链反应测定肝脏ET-1 mRNA表达，并使用酶联免疫吸附测定法评估肝脏和血浆ET-1水平。通过腹腔镜测量脾髓压力（SPP），作为门静脉压力的指标。
患CH的犬的肝脏ET-1 mRNA水平比健康犬高3.7倍（P = .008）。患CH的犬的肝脏和血浆ET-1蛋白水平中位数显著高于健康犬（湿肝组织分别为13.20 pg/mg vs. 3.42 pg/mg，P = .004；血浆分别为0.99 pg/mL vs. 0.71 pg/mL，P = .013）。此外，患CH的犬的血浆ET-1水平与SPP之间存在弱但显著的相关性（P = .036；r = 0.53）。
结果表明，ET-1可能在CH所致门静脉高压的发病机制中起重要作用。
