State Key Laboratory of Biochemical Engineering, CAS Center for Excellence in Nanoscience, Institute of Process Engineering, Chinese Academy of Sciences, No. 1 Beiertiao, Zhongguancun, Beijing, 100190, P. R. China.
Department of Physical Chemistry, School of Metallurgical and Ecological Engineering, University of Science and Technology Beijing, No. 30, Xueyuan Road, Haidian District, Beijing, 100083, P. R. China.
Adv Mater. 2017 May;29(18). doi: 10.1002/adma.201606755. Epub 2017 Mar 10.
Despite recent progress in 2D nanomaterials-based biosensing, it remains challenging to achieve sensitive and high selective detection. This study develops few-layer graphdiyne (GD) nanosheets (NSs) that are used as novel sensing platforms for a variety of fluorophores real-time detection of DNA with low background and high signal-to-noise ratio, which show a distinguished fluorescence quenching ability and different affinities toward single-stranded DNA and double-stranded DNA. Importantly, for the first time, a few-layer GD NSs-based multiplexed DNA sensor is developed.
尽管基于 2D 纳米材料的生物传感技术最近取得了进展，但实现灵敏和高选择性的检测仍然具有挑战性。本研究开发了少层石墨炔（GD）纳米片（NSs），将其用作新型传感平台，可实现各种荧光团的实时检测，具有低背景和高信噪比，对单链 DNA 和双链 DNA 表现出出色的荧光猝灭能力和不同的亲和力。重要的是，首次开发了基于少层 GD NSs 的多重 DNA 传感器。
