So Eui Young, Winchester Trisha, Ouchi Toru
a Deparment of Cancer Genetics , Roswell Park Cancer Institute , Buffalo , NY , USA.
Cancer Biol Ther. 2017 Mar 4;18(3):152-157. doi: 10.1080/15384047.2017.1281498.
There is increasing evidence showing specific roles of microRNA in cell differentiation and cancer progression. Here we examine miRNA profiles during maturation of monocytes and bone marrow-derived dendritic cells (BMDCs) in human and mouse, respectively. We have identified significant changes of various miRNA expression during monocyte and BMDC monocyte development via miRNA microarrays, confirmed by quantitative PCR. Increases in miR155 expression positively correlated with increasing maturity of monocyte and BMDC in both mouse and human microarrays, indicating its importance in development. We describe a requirement of miR155 for MHCII expression during GM-CSF-induced development and LPS-induced maturation of DCs, suggesting reduced immune function of DC when miR155 is absent. Our study suggests that miRNAs might have an important role in differentiation of myeloid cell such as dendritic cells and macrophages.
越来越多的证据表明，微小RNA在细胞分化和癌症进展中发挥着特定作用。在此，我们分别研究了人和小鼠单核细胞及骨髓来源的树突状细胞（BMDC）成熟过程中的微小RNA谱。通过微小RNA微阵列，我们鉴定出了单核细胞和BMDC发育过程中各种微小RNA表达的显著变化，并通过定量PCR进行了验证。在人和小鼠微阵列中，miR155表达的增加与单核细胞和BMDC成熟度的增加呈正相关，表明其在发育中的重要性。我们描述了在GM-CSF诱导的DC发育和LPS诱导的DC成熟过程中，miR155对MHCII表达的需求，这表明当miR155缺失时DC的免疫功能会降低。我们的研究表明，微小RNA可能在髓样细胞如树突状细胞和巨噬细胞的分化中发挥重要作用。
