Rivera-Piza Adriana, An Young Jae, Kim Do Kyung, Lee Sung-Hyen, Kim Jung-Bong, Choi Jung-Sook, Lee Sung-Joon
1 Department of Biotechnology, Graduate School of Life Sciences and Biotechnology, Korea University , Seoul, Korea.
2 National Academy of Agricultural Science, Rural Development Administration , Wanju Gun, Korea.
J Med Food. 2017 Mar;20(3):309-319. doi: 10.1089/jmf.2016.3833.
Abnormal activation of adipogenesis in mesenchymal stem cells (MSCs) and preadipocyte cells is associated with human metabolic disorders, such as osteoporosis and obesity. This study investigated the biological effects of protocatechuic acid (PCA) on the modulation of osteogenesis and adipogenesis in cultured cells. PCA stimulation of MSCs significantly increased intracellular mineralization during osteogenesis, but reduced lipid accumulation in both MSCs and 3T3-L1 preadipocyte cells during adipogenesis. Reverse transcription-polymerase chain reaction and immunoblotting analyses showed a dose-dependent upregulation of proosteogenic runt-related transcription factor 2 due to induction of β-catenin. PCA reduced the expression of proadipogenic transcription factor, peroxisome proliferator-activated receptor-γ, and suppressed its promotor activity. These results suggest PCA exerts stimulatory effects on the osteogenesis of MSCs and inhibitory effects on the adipogenesis of MSCs and 3T3-L1 cells. PCA may contribute to maintain a coordinated metabolic balance between adipogenesis and osteogenesis, and thus may be useful for the prevention and alleviation of osteoporosis and obesity.
间充质干细胞（MSCs）和前脂肪细胞中脂肪生成的异常激活与人类代谢紊乱有关，如骨质疏松症和肥胖症。本研究调查了原儿茶酸（PCA）对培养细胞中骨生成和脂肪生成调节的生物学效应。在成骨过程中，PCA刺激MSCs显著增加细胞内矿化，但在脂肪生成过程中，PCA减少了MSCs和3T3-L1前脂肪细胞中的脂质积累。逆转录-聚合酶链反应和免疫印迹分析表明，由于β-连环蛋白的诱导，促骨生成的 runt相关转录因子2呈剂量依赖性上调。PCA降低了促脂肪生成转录因子过氧化物酶体增殖物激活受体γ的表达，并抑制了其启动子活性。这些结果表明，PCA对MSCs的成骨具有刺激作用，对MSCs和3T3-L1细胞的脂肪生成具有抑制作用。PCA可能有助于维持脂肪生成和骨生成之间协调的代谢平衡，因此可能对预防和缓解骨质疏松症和肥胖症有用。
