Herbal Medicine Research Division, Korea Institute of Oriental Medicine, Yuseong-daero 1672, Yuseong-gu, Daejeon 34054, Korea.
Molecules. 2019 Aug 22;24(17):3051. doi: 10.3390/molecules24173051.
In traditional oriental medicine, Nakaike is widely used in treating bone diseases. Postmenopausal women are strongly associated with osteoporosis and obesity. This study aimed to investigate the effects of the water extract of (WDR) on bone loss and obesity in ovariectomized (OVX) mice fed a high-fat diet (HFD). Body weight, gonadal fat weight, histological findings, and morphometric parameters in trabecular bone were evaluated after OVX mice were treated with WDR and HFD for four weeks. The receptor activator of nuclear κ-B ligand (RANKL)-induced osteoclast differentiation in bone marrow-derived macrophages (BMMs) was examined. Phytochemical identification of WDR using ultrahigh-performance liquid chromatography-tandem mass spectrometry was performed. WDR reversed the changes in body weight gain, gonadal fat mass, and trabecular bone parameters by ovariectomy. However, ovariectomy-induced uterine atrophy was not affected by WDR. WDR decreased adipocyte size and pro-inflammatory cytokines (interleukin (IL)-1β and IL-6) in gonadal fats and lipid accumulation in the bone marrow, which were induced by ovariectomy. WDR significantly decreased RANKL-induced osteoclast differentiation in BMMs. Fifteen phytochemicals were identified in WDR: Seven and nine with anti-osteoporotic and anti-adipogenic activities, respectively. Our findings suggest that WDR may have beneficial effects on postmenopausal osteoporosis and obesity.
在传统的东方医学中，中濑（Nakaike）被广泛用于治疗骨骼疾病。绝经后妇女与骨质疏松症和肥胖症密切相关。本研究旨在探讨（WDR）水提取物对高脂饮食（HFD）喂养去卵巢（OVX）小鼠骨丢失和肥胖的影响。在 HFD 喂养四周后，用 WDR 处理 OVX 小鼠，评估其体重、性腺脂肪重量、组织学发现和小梁骨的形态计量学参数。检测了核因子 κ-B 配体（RANKL）诱导的骨髓源性巨噬细胞（BMM）破骨细胞分化。采用超高效液相色谱-串联质谱法对 WDR 进行了植物化学成分鉴定。WDR 逆转了去卵巢引起的体重增加、性腺脂肪量和小梁骨参数的变化。然而，WDR 对去卵巢引起的子宫萎缩没有影响。WDR 减少了性腺脂肪中的脂肪细胞大小和促炎细胞因子（白细胞介素（IL）-1β和 IL-6）以及骨髓中的脂质积累，这些都是由去卵巢引起的。WDR 显著减少了 BMM 中 RANKL 诱导的破骨细胞分化。在 WDR 中鉴定出 15 种植物化学成分：分别有 7 种和 9 种具有抗骨质疏松和抗脂肪生成活性。我们的研究结果表明，WDR 可能对绝经后骨质疏松症和肥胖症有有益的影响。
