印度西南海岸芒格洛尔蛤类样本中临床基因型的出现情况。
Occurrence of clinical genotype in clam samples in Mangalore, Southwest coast of India.
作者信息
Sangeetha M S, Shekar Malathi, Venugopal M N
机构信息
Department of Fisheries Microbiology, College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University, Mangalore, 575 002 India.
出版信息
J Food Sci Technol. 2017 Mar;54(3):786-791. doi: 10.1007/s13197-017-2522-y. Epub 2017 Feb 15.
Abstract
is an opportunistic human pathogen causing gastroenteritis, wound infection and primary septicemia. population has been divided into subpopulations based on their phenotype and genotype characteristics. In this study, 38.5% (10/26) of clam () samples obtained from Mangalore markets were seen to harbor Biochemical characterization of isolates showed the strains to belong to Biotype 1 phenotype. Genotyping of strains using the 16S rRNA and virulence correlated gene () typing methods identified the isolates to be of 16S rRNA typeB and C type respectively. Analysis of representative 16S rRNA and gene sequences further substantiated that the associated with clams in the present study to be of clinical origin, implicated as virulent type responsible for causing infection in humans.
摘要
是一种机会性人类病原体，可引起肠胃炎、伤口感染和原发性败血症。根据其表型和基因型特征，该种群已被分为亚种群。在本研究中，从芒格洛尔市场采集的38.5%（10/26）的蛤（）样本被发现携带 分离株的生化特征表明这些菌株属于生物型1表型。使用16S rRNA和毒力相关基因（）分型方法对菌株进行基因分型，确定分离株分别为16S rRNA B型和C型。对代表性的16S rRNA和基因序列的分析进一步证实，本研究中与蛤相关的 来自临床来源，被认为是导致人类感染的致病类型。
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