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Detection and quantification of Vibrio parahaemolyticus in shellfish from Italian production areas.检测和定量分析来自意大利产区贝类中的副溶血性弧菌。
Int J Food Microbiol. 2014 Aug 1;184:14-20. doi: 10.1016/j.ijfoodmicro.2014.04.016. Epub 2014 Apr 21.
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Vibrio fluvialis: an emerging human pathogen.河流弧菌：一种新出现的人类病原体。
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A new culture-based method for the improved identification of Vibrio vulnificus from environmental samples, reducing the need for molecular confirmation.一种新的基于培养的方法可提高从环境样本中鉴定创伤弧菌的能力，减少对分子确认的需求。
J Microbiol Methods. 2013 Jun;93(3):277-83. doi: 10.1016/j.mimet.2013.03.023. Epub 2013 Apr 6.
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意大利艾米利亚-罗马涅和撒丁岛的蛤蜊（亚当斯和里夫，1850年）中弧菌、弧菌和弧菌的存在情况。

Occurrence of Vibrio , Vibrio and in the Clam (Adams & Reeve, 1850) from Emilia Romagna and Sardinia, Italy.

作者信息

Passalacqua Pier Luca, Zavatta Emanuele, Bignami Giorgia, Serraino Andrea, Serratore Patrizia

机构信息

Department of Veterinary Medical Science, Alma Mater Studiorum-University of Bologna , Ozzano Emilia (BO), Italy.

出版信息

Ital J Food Saf. 2016 Apr 1;5(1):5709. doi: 10.4081/ijfs.2016.5709. eCollection 2016 Jan 18.

DOI:10.4081/ijfs.2016.5709
PMID:27800436
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5076712/
Abstract

Marine vibrios, , and are responsible of the majority of food-borne human infections by consumption of bivalve shellfish. The aim of the present study was to ascertain the occurrence of these bacteria, and their potential pathogenicity, in the Manila clam from Emilia Romagna (ER) and Sardinia (SR) regions, Italy. Isolation was performed on CHROMagar vibrio with subculture on (thiosulfate-citrate-bile salts-sucrose) Agar and m-modified-cellobiose-polymyxin b-colistin (-CPC) Agar. Suspected strains were purified, biochemically characterized and genotyped by simplex polymerase chain reaction (PCR) for the specie-specific and pathogenic gene markers: (P, and ); . (, , C, E, CPS operon allele 1, CPS operon allele 2, 16s-rRNA operon allele A, 16s-rRNA operon allele B; (C, , , , , , /). Moreover a multiplex PCR was applied to the SR bivalve shellfish, for the simultaneous detection of the three targets directly on homogenate samples, targeting the species-specific gene for (C), (P) and (). As a result of phenotyping and genotyping of isolates, bivalve shellfish from ER resulted positive for . (27.8%) and (10.1%), but negative for . Shellfish from SR resulted positive for . (30.3%), (6.1%) and (3%). No significant differences emerged between the two areas (P>0.05).

摘要

海洋弧菌、 、 是食用双壳贝类导致大多数食源性人类感染的罪魁祸首。本研究的目的是确定这些细菌在意大利艾米利亚-罗马涅（ER）和撒丁岛（SR）地区的马尼拉蛤中的存在情况及其潜在致病性。在CHROMagar弧菌培养基上进行分离，并在（硫代硫酸盐-柠檬酸盐-胆盐-蔗糖）琼脂和m-改良纤维二糖-多粘菌素b-黏菌素（-CPC）琼脂上进行传代培养。对疑似菌株进行纯化，通过单重聚合酶链反应（PCR）对其进行生化特征鉴定和基因分型，以检测种特异性和致病基因标记： （P、 和 ）； 。 （ 、 、C、E、CPS操纵子等位基因1、CPS操纵子等位基因2、16s-rRNA操纵子等位基因A、16s-rRNA操纵子等位基因B； （C、 、 、 、 、 、 /）。此外，对SR双壳贝类应用多重PCR，直接在匀浆样品上同时检测三个靶标，靶向 （C）、 （P）和 （）的种特异性基因。对分离株进行表型和基因分型的结果显示，来自ER的双壳贝类 （27.8%）和 （10.1%）检测呈阳性，但 检测呈阴性。来自SR的贝类 （30.3%）、 （6.1%）和 （3%）检测呈阳性。两个地区之间未出现显著差异（P>0.05）。