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在小鼠怀孕期间，条件性胰岛低血管化并不妨碍β细胞的扩张。

Conditional islet hypovascularisation does not preclude beta cell expansion during pregnancy in mice.

机构信息

Beta Cell Neogenesis (BENE), Vrije Universiteit Brussel, Laarbeeklaan 103, 1090, Brussels, Belgium.

Department of Paediatrics, Division of Paediatric Endocrinology, Ghent University Hospital and Ghent University, Ghent, Belgium.

出版信息

Diabetologia. 2017 Jun;60(6):1051-1056. doi: 10.1007/s00125-017-4243-1. Epub 2017 Mar 16.

DOI:10.1007/s00125-017-4243-1
PMID:28299380
Abstract

AIMS/HYPOTHESIS: Endothelial-endocrine cell interactions and vascular endothelial growth factor (VEGF)-A signalling are deemed essential for maternal islet vascularisation, glucose control and beta cell expansion during mouse pregnancy. The aim of this study was to assess whether pregnancy-associated beta cell expansion was affected under conditions of islet hypovascularisation.

METHODS

Soluble fms-like tyrosine kinase 1 (sFLT1), a VEGF-A decoy receptor, was conditionally overexpressed in maternal mouse beta cells from 1.5 to 14.5 days post coitum. Islet vascularisation, glycaemic control, beta cell proliferation, individual beta cell size and total beta cell volume were assessed in both pregnant mice and non-pregnant littermates.

RESULTS

Conditional overexpression of sFLT1 in beta cells resulted in islet hypovascularisation and glucose intolerance in both pregnant and non-pregnant mice. In contrast to non-pregnant littermates, glucose intolerance in pregnant mice was transient. sFLT1 overexpression did not affect pregnancy-associated changes in beta cell proliferation, individual beta cell size or total beta cell volume.

CONCLUSIONS/INTERPRETATION: Reduced intra-islet VEGF-A signalling results in maternal islet hypovascularisation and impaired glycaemic control but does not preclude beta cell expansion during mouse pregnancy.

摘要

目的/假设：内皮-内分泌细胞相互作用和血管内皮生长因子（VEGF）-A 信号对于母鼠胰岛血管生成、葡萄糖控制和β细胞扩张至关重要。本研究旨在评估在胰岛血管生成不足的情况下，妊娠相关的β细胞扩张是否受到影响。

方法

从妊娠 1.5 天到 14.5 天，条件性过表达可溶性 fms 样酪氨酸激酶 1（sFLT1），一种 VEGF-A 诱饵受体，在母鼠β细胞中。在怀孕小鼠和未怀孕的同窝仔鼠中评估胰岛血管生成、血糖控制、β细胞增殖、单个β细胞大小和总β细胞体积。

结果

β细胞中 sFLT1 的条件性过表达导致胰岛血管生成不足和无论是否怀孕的小鼠的葡萄糖耐量受损。与未怀孕的同窝仔鼠不同，怀孕小鼠的葡萄糖耐量受损是短暂的。sFLT1 的过表达并不影响妊娠相关的β细胞增殖、单个β细胞大小或总β细胞体积的变化。

结论/解释：减少胰岛内 VEGF-A 信号转导导致母鼠胰岛血管生成不足和血糖控制受损，但不排除小鼠妊娠期间β细胞扩张。

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本文引用的文献

1
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2
Gestational Diabetes Mellitus From Inactivation of Prolactin Receptor and MafB in Islet β-Cells.胰岛β细胞中催乳素受体和MafB失活导致的妊娠期糖尿病
Diabetes. 2016 Aug;65(8):2331-41. doi: 10.2337/db15-1527. Epub 2016 May 23.
3
Phenotypic Characterization of MIP-CreERT1Lphi Mice With Transgene-Driven Islet Expression of Human Growth Hormone.
Diabetologia. 2019 Nov;62(11):1961-1968. doi: 10.1007/s00125-019-4969-z. Epub 2019 Aug 14.
4
Decreased -Cell Proliferation and Vascular Density in a Subpopulation of Low-Oxygenated Male Rat Islets.低氧雄性大鼠胰岛亚群中细胞增殖和血管密度降低
J Endocr Soc. 2019 Jun 17;3(8):1608-1616. doi: 10.1210/js.2019-00101. eCollection 2019 Aug 1.
5
Vegf-A mRNA transfection as a novel approach to improve mouse and human islet graft revascularisation.Vegf-A mRNA 转染作为一种提高鼠和人胰岛移植物再血管化的新方法。
Diabetologia. 2018 Aug;61(8):1804-1810. doi: 10.1007/s00125-018-4646-7. Epub 2018 May 22.
6
Vascular-derived connective tissue growth factor (Ctgf) is critical for pregnancy-induced β cell hyperplasia in adult mice.血管源性结缔组织生长因子（Ctgf）对成年小鼠妊娠诱导的β细胞增生至关重要。
Islets. 2017 Nov 2;9(6):150-158. doi: 10.1080/19382014.2017.1356963. Epub 2017 Nov 7.
具有转基因驱动人生长激素胰岛表达的MIP-CreERT1Lphi小鼠的表型特征
Diabetes. 2015 Nov;64(11):3798-807. doi: 10.2337/db15-0272. Epub 2015 Jul 7.
4
Impaired islet function in commonly used transgenic mouse lines due to human growth hormone minigene expression.由于人类生长激素小基因的表达，常用转基因小鼠品系的胰岛功能受损。
Cell Metab. 2014 Dec 2;20(6):979-90. doi: 10.1016/j.cmet.2014.11.004.
5
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6
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Diabetes. 2013 Dec;62(12):4165-73. doi: 10.2337/db12-1827. Epub 2013 Aug 23.
7
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8
Vascular instruction of pancreas development.血管对胰腺发育的指导作用。
Development. 2012 Aug;139(16):2833-43. doi: 10.1242/dev.065953.
9
Fiji: an open-source platform for biological-image analysis.斐济：一个用于生物影像分析的开源平台。
Nat Methods. 2012 Jun 28;9(7):676-82. doi: 10.1038/nmeth.2019.
10
The hypoxia response pathway and β-cell function.缺氧反应通路与β细胞功能。
Diabetes Obes Metab. 2010 Oct;12 Suppl 2:159-67. doi: 10.1111/j.1463-1326.2010.01276.x.