Yang J W, Tian G, Chen D W, Yao Y, He J, Zheng P, Mao X B, Yu J, Huang Z Q, Yu B
Institute of Animal Nutrition, Sichuan Agricultural University, Yaan, Sichuan, China.
J Anim Physiol Anim Nutr (Berl). 2018 Feb;102(1):252-259. doi: 10.1111/jpn.12686. Epub 2017 Mar 16.
Weaning is characterized by intestinal inflammation, which is a big challenge in pig industry. Control of intestinal inflammation is important for improvement of growth performance and health. Therefore, the study was focused on the anti-inflammatory activity of low-molecular-weight chitosan oligosaccharide (LCOS) in a porcine small intestinal epithelial cell line (IPEC-J2). The results showed that TNF-α, as inflammation inducer, significantly upregulated the mRNA expression of IL-8 and MCP-1. Afterwards, LCOS significantly attenuated mRNA expression of IL-8 and MCP-1 induced by TNF-α in the cells. Mannose (MAN), as ligand of mannose receptor, had no effect on the anti-inflammatory activity of LCOS, which suggested that mannose receptor may not involve in the anti-inflammatory activity of LCOS in IPEC-J2 cells. Interestingly, N-[2-(p-bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide 2HCl hydrate (H89), as PKA (protein kinase A)-specific inhibitor, reversed the mRNA expression of IL-8 when co-cultured with LCOS. Furthermore, LCOS concentration dependent downregulated the mRNA expression of claudin-1 compared with TNF-α treatment. However, the trans-epithelial electric resistance (TEER) was not affected by LCOS when co-cultured with TNF-α in 3 hr. In conclusion, LCOS have a potent anti-inflammatory activity, and as a feed additives, may be useful for the inhibition of inflammatory process in weaning period of pigs with intestinal inflammation occurring.
断奶的特征是肠道炎症，这是养猪业面临的一大挑战。控制肠道炎症对于提高生长性能和健康状况至关重要。因此，本研究聚焦于低分子量壳寡糖（LCOS）在猪小肠上皮细胞系（IPEC-J2）中的抗炎活性。结果表明，作为炎症诱导剂的TNF-α显著上调了IL-8和MCP-1的mRNA表达。随后，LCOS显著减弱了TNF-α诱导的细胞中IL-8和MCP-1的mRNA表达。甘露糖（MAN）作为甘露糖受体的配体，对LCOS的抗炎活性没有影响，这表明甘露糖受体可能不参与LCOS在IPEC-J2细胞中的抗炎活性。有趣的是，作为蛋白激酶A（PKA）特异性抑制剂的N-[2-（对溴肉桂氨基）乙基]-5-异喹啉磺酰胺二盐酸盐水合物（H89）与LCOS共培养时可逆转IL-8的mRNA表达。此外，与TNF-α处理相比，LCOS浓度依赖性地下调了claudin-1的mRNA表达。然而，与TNF-α共培养3小时时，LCOS对跨上皮电阻（TEER）没有影响。总之，LCOS具有强大的抗炎活性，作为饲料添加剂，可能有助于抑制断奶期发生肠道炎症的仔猪的炎症过程。
