Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, China.
Molecules. 2021 Jan 22;26(3):569. doi: 10.3390/molecules26030569.
Low-molecular-weight chitosan (LMWC), a product of chitosan deacetylation, possesses anti-inflammatory effects. In the present study, a porcine small intestinal epithelial cell line, IPEC-J2, was used to assess the protective effects of LMWC on lipopolysaccharide (LPS)-induced intestinal epithelial cell injury. IPEC-J2 cells were pretreated with or without LMWC (400 μg/mL) in the presence or absence of LPS (5 μg/mL) for 6 h. LMWC pretreatment increased ( < 0.05) the occludin abundance and decreased ( < 0.05) the tumour necrosis factor-α (TNF-α) production, apoptosis rate and cleaved cysteinyl aspartate-specific protease-3 (caspase-3) and -8 contents in LPS-treated IPEC-J2 cells. Moreover, LMWC pretreatment downregulated ( < 0.05) the expression levels of TNF receptor 1 () and TNFR-associated death domain and decreased ( < 0.05) the nuclear and cytoplasmic abundance of nuclear factor-κB (NF-κB) p65 in LPS-stimulated IPEC-J2 cells. These results suggest that LMWC exerts a mitigation effect on LPS-induced intestinal epithelial cell damage by suppressing TNFR1-mediated apoptosis and decreasing the production of proinflammatory cytokines via the inhibition of NF-κB signalling pathway.
低分子量壳聚糖（LMWC）是壳聚糖脱乙酰化的产物，具有抗炎作用。本研究采用猪小肠上皮细胞系 IPEC-J2 来评估 LMWC 对脂多糖（LPS）诱导的肠道上皮细胞损伤的保护作用。将 IPEC-J2 细胞用或不用 LPS（5μg/mL）预处理 6 小时，同时用或不用 LMWC（400μg/mL）预处理。LMWC 预处理增加（<0.05）了 LPS 处理的 IPEC-J2 细胞中紧密连接蛋白（occludin）的丰度，并降低（<0.05）了肿瘤坏死因子-α（TNF-α）的产生、细胞凋亡率、半胱天冬氨酸特异性蛋白酶-3（caspase-3）和 -8 的裂解片段含量。此外，LMWC 预处理下调（<0.05）了 TNF 受体 1（）和 TNFR 相关死亡结构域的表达水平，并降低（<0.05）了 LPS 刺激的 IPEC-J2 细胞中核因子-κB（NF-κB）p65 的核内和胞质丰度。这些结果表明，LMWC 通过抑制 TNFR1 介导的细胞凋亡和抑制 NF-κB 信号通路来减少促炎细胞因子的产生，从而对 LPS 诱导的肠道上皮细胞损伤发挥缓解作用。
