Department of Floriculture, Ornamental Horticulture and Garden Design, Faculty of Agriculture (El-Shatby), Alexandria University, Alexandria, Egypt.
Department of Plant Pathology, Faculty of Agriculture (ELShatby), Alexandria University, Alexandria, Egypt.
Microb Pathog. 2017 Dec;113:348-356. doi: 10.1016/j.micpath.2017.11.004. Epub 2017 Nov 7.
Three bacterial isolates were isolated from infected potato tubers showing soft and brown rots like symptoms as well as one isolate from infected peach tree showing crown gall symptom. The morphological, biochemical and molecular assays proved that bacterial isolates belonging to Pectobacterium carotovorum subsp. carotovorum, Ralstonia solanacearum, Dickeya spp. and Agrobacterium tumefaciens. The acetone (AcE) and n-butanol (ButE) extracts of Callistemon viminalis flowers and essential oil from aerial parts of Conyza dioscoridis as well as ButE of Eucalyptus camaldulensis bark are evaluated at different concentrations against the growth of the isolated bacteria. The diameter of inhibition zone (IZ) and the minimum inhibitory concentrations (MICs) are compared. Results indicated that the highest IZ values were 20.0 mm and 18.3 mm for E. camaldulensis bark ButE and C. viminalis flower ButE, respectively, against P. carotovorum; 16.3 mm and 16.0 mm for E. camaldulensis bark ButE and C. viminalis flower ButE, respectively, against R. solanacearum; 18.5 mm for C. viminalis flower AcE and C. dioscoridis aerial parts EO against Dickeya spp.; and 15.0 mm for C. viminalis flower AcE against A. tumefaciens. MICs ranged from <16 μg/mL for D. solani to >4000 μg/mL for A. tumefaciens. It was proved that C. viminalis flowers AcE contains mainly 5-hydroxymethylfurfural (20.6%), palmitic acid (18.5%), and pyrogallol (16.4%); while C. viminalis flower ButE contains palmitic acid (36.3%), 2-hydroxymyristic acid (9.4%), 5-hydroxymethylfurfural (7.2%), and shikimic acid (6.6%); whereas E. camaldulensis bark ButE contains 8-nonynoic acid methyl ester (45.6), camphor (30.9%), menthol (8.8%), and 1,8-cineole (eucalyptol) (8.2%), whilst the EO of C. dioscoridis aerial parts comprises Z-(13,14-epoxy)tetradec-11-en-1-ol acetate (11.6%), γ-elemene (10.2%), tau.-muurolol (7.1%), and cadina-3,9-diene (4.7%). It can be concluded that phytochemical extracts of C. viminalis, E. camaldulensis and C. dioscoridis demonstrated strong to moderate antibacterial effects against the studied plant bacterial pathogens.
从表现出软腐和棕色腐烂症状的感染马铃薯块茎中分离出三个细菌分离株，以及一个表现出冠瘿症状的感染桃树分离株。形态学、生化和分子分析证明，细菌分离株属于胡萝卜软腐果胶杆菌亚种、茄科劳尔氏菌、菊科食酸菌和根癌农杆菌。在不同浓度下，评估了金合欢花的丙酮（AcE）和正丁醇（ButE）提取物、斑鸠菊地上部分的精油以及桉树树皮的正丁醇提取物对分离细菌的生长的抑制作用。比较了抑菌圈（IZ）直径和最小抑菌浓度（MICs）。结果表明，对胡萝卜软腐果胶杆菌的最高 IZ 值分别为桉树树皮正丁醇提取物 20.0mm 和金合欢花正丁醇提取物 18.3mm；对茄科劳尔氏菌的最高 IZ 值分别为桉树树皮正丁醇提取物 16.3mm 和金合欢花正丁醇提取物 16.0mm；对食酸菌的最高 IZ 值为金合欢花 AcE 和斑鸠菊地上部分 EO，分别为 18.5mm；对根癌农杆菌的最高 IZ 值为金合欢花 AcE，为 15.0mm。MIC 范围从<16μg/mL 到>4000μg/mL 不等，具体取决于不同的细菌分离株。证明金合欢花 AcE 主要含有 5-羟甲基糠醛（20.6%）、棕榈酸（18.5%）和焦儿茶酚（16.4%）；而金合欢花正丁醇提取物含有棕榈酸（36.3%）、2-羟葵酸（9.4%）、5-羟甲基糠醛（7.2%）和莽草酸（6.6%）；桉树树皮正丁醇提取物含有 8-壬炔酸甲酯（45.6%）、樟脑（30.9%）、薄荷醇（8.8%）和 1,8-桉树脑（桉树脑）（8.2%），而斑鸠菊地上部分的精油则含有 Z-（13,14-环氧）十四碳-11-烯-1-醇乙酸酯（11.6%）、γ-榄香烯（10.2%）、τ-杜松醇（7.1%）和卡达-3,9-二烯（4.7%）。可以得出结论，金合欢、桉树和斑鸠菊的植物化学提取物对研究的植物病原菌具有较强至中度的抗菌作用。
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