Elansary Hosam O, Yessoufou' Kowiyou, Mahmoud Eman A, Skalicka-Woźniak Krystyna
Nat Prod Commun. 2016 Oct;11(10):1455-1458.
Bioactive compounds, including phenols, flavonoids, and tannins, were quantified in leaves, stems and roots of methanol, n-butanol, diethyl ether and n- hexane extracts of Ceratostigina plumbaginoides Bunge. (Plumbaginaceae) ornamental plants. The antioxidant capacity was measured by the DPPH and linoleic acid assays. The total bioactive compounds, as well as the antioxidant capacities, were the highest in the leaves compared with stems and roots. The -methanolic, n-butanol, diethyl ether and n-hexane leaf extracts varied in their antibacterial and antifungal activities. In general, the most sensitive bacterium to leaf extracts was Bacillus cereus and the most resistant was Staphyllococcus aureus, while the most sensitive fungus was Aspergillusflavus and the most resistant one was Penicillium ochrochloron. As the methanolic leaf extract was the most active, it was subjected to column chromatography and two compounds were isolated and identified as 1 (5-hydroxy-2-methyl-1,4-naphthoquinone / plumbagin) and 2 (3,3'-biplumbagin). Compounds 1 and 2 showed the highest antibacterial and antifungal activities compared with other extracts tested. The MIC and MBC values for the most active compound 1 were in the range of 0.001 - 0.09 and 0.004 - 0.21 mg mL⁻¹, while MIC and MFC were determined as 0.001 - 0.11 and 0.002 - 0.19 mg mL⁻¹, respectively. The isolated compounds and leaf extracts showed also equal or higher antimicrobial activities compared with antibiotics/commercial reagents which indicate that the plant might be useful for drug development. This is the first report on the antibacterial and antifungal activities, as well as the antioxidant properties of the tested plant parts and isolated compounds.
对蓝雪花（蓝雪科）观赏植物的甲醇、正丁醇、乙醚和正己烷提取物的叶、茎和根中的生物活性化合物（包括酚类、黄酮类和单宁）进行了定量分析。通过DPPH和亚油酸测定法测量抗氧化能力。与茎和根相比，叶中的总生物活性化合物以及抗氧化能力最高。甲醇、正丁醇、乙醚和正己烷叶提取物的抗菌和抗真菌活性各不相同。一般来说，对叶提取物最敏感的细菌是蜡样芽孢杆菌，最具抗性的是金黄色葡萄球菌，而最敏感的真菌是黄曲霉，最具抗性的是淡绿青霉。由于甲醇叶提取物活性最强，对其进行了柱色谱分离，分离并鉴定出两种化合物，分别为1（5-羟基-2-甲基-1,4-萘醌/白花丹素）和2（3,3'-联白花丹素）。与其他测试提取物相比，化合物1和2表现出最高的抗菌和抗真菌活性。活性最强的化合物1的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）值在0.001 - 0.09和0.004 - 0.21 mg mL⁻¹范围内，而最低抑菌浓度（MIC）和最低杀菌浓度（MFC）分别测定为0.001 - 0.11和0.002 - 0.19 mg mL⁻¹。分离出的化合物和叶提取物与抗生素/商业试剂相比，也表现出同等或更高的抗菌活性，这表明该植物可能对药物开发有用。这是关于受试植物部位和分离化合物的抗菌和抗真菌活性以及抗氧化特性的首次报道。
