马来西亚鸡肉中分离的多药耐药性肠炎沙门氏菌血清型 Brancaster 菌株 PS01 的基因组草图
Draft genome sequence of multidrug-resistant Salmonella enterica serovar Brancaster strain PS01 isolated from chicken meat, Malaysia.
机构信息
Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia; Integrative Pharmacogenomics Institute (iPROMISE), Universiti Teknologi MARA, Puncak Alam Campus, 42300 Bandar Puncak Alam, Selangor, Malaysia.
出版信息
J Glob Antimicrob Resist. 2017 Jun;9:41-42. doi: 10.1016/j.jgar.2016.12.017. Epub 2017 Mar 12.
OBJECTIVES
Salmonella spp. represent one of the main diarrhoeal pathogens that are transmitted via the food supply chain. Here we report the draft genome sequence of a multidrug-resistant Salmonella enterica serovar Brancaster (PS01) that was isolated from poultry meat in Malaysia.
METHODS
Genomic DNA was extracted from Salmonella strain PS01 and was sequenced using an Illumina HiSeq 2000 platform. The generated reads were de novo assembled using CLC Genomics Workbench. The draft genome was annotated and the presence of antimicrobial resistance genes was identified.
RESULTS
The 5 036 442bp genome contains various antimicrobial resistance genes conferring resistance to aminoglycosides, fluoroquinolones, fosfomycin, macrolides, phenicols, sulphonamides, tetracyclines and trimethoprim. The β-lactamase gene bla encoding TEM-176 was also found in this strain.
CONCLUSIONS
The genome sequence will aid in the understanding of drug resistance mechanisms in foodborne Salmonella Brancaster and highlights the need to ensure the judicious use of antibiotics in animal husbandry as well as the importance of implementing proper food handling and preparation practices.
目的
沙门氏菌属是通过食物链传播的主要腹泻病原体之一。本研究报告了从马来西亚禽肉中分离出的多重耐药性肠炎沙门氏菌血清型布兰卡斯特（PS01）的基因组序列草案。
方法
从沙门氏菌 PS01 菌株中提取基因组 DNA，并使用 Illumina HiSeq 2000 平台进行测序。使用 CLC Genomics Workbench 对生成的读数进行从头组装。对基因组草案进行注释，并鉴定出存在的抗生素耐药基因。
结果
该 5036442bp 基因组包含各种抗生素耐药基因，可赋予对氨基糖苷类、氟喹诺酮类、磷霉素、大环内酯类、酚类、磺胺类、四环素类和甲氧苄啶的耐药性。该菌株还存在编码 TEM-176 的β-内酰胺酶基因 bla。
结论
该基因组序列将有助于了解食源性病原体肠炎沙门氏菌布兰卡斯特的耐药机制，并强调需要确保在畜牧业中合理使用抗生素，以及实施适当的食物处理和准备措施的重要性。
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