Kim Dong-Ho, Hong Sung-Yong, Kang Jea Woo, Cho Sung Min, Lee Kyu Ri, An Tae Kyung, Lee Chan, Chung Soo Hyun
National Agricultural Products Quality Management Service, 5-3 Gimcheon innocity, Nam-myeon, Gimcheon City Gyeongsangbuk-do Province 39660, Korea.
Department of Integrated Biomedical and Life Science, Korea University, Seoul 02841, Korea.
Toxins (Basel). 2017 Mar 16;9(3):106. doi: 10.3390/toxins9030106.
An improved analytical method compared with conventional ones was developed for simultaneous determination of 13 mycotoxins (deoxynivalenol, nivalenol, 3-acetylnivalenol, aflatoxin B₁, aflatoxin B₂, aflatoxin G₁, aflatoxin G₂, fumonisin B₁, fumonisin B₂, T-2, HT-2, zearalenone, and ochratoxin A) in cereal grains by liquid chromatography-tandem mass spectrometry (LC/MS/MS) after a single immunoaffinity column clean-up. The method showed a good linearity, sensitivity, specificity, and accuracy in mycotoxin determination by LC/MS/MS. The levels of 13 mycotoxins in 5 types of commercial grains (brown rice, maize, millet, sorghum, and mixed cereal) from South Korea were determined in a total of 507 cereal grains. Mycotoxins produced from sp. (fumonisins, deoxynivalenol, nivalenol, and zearalenone) were more frequently (more than 5%) and concurrently detected in all cereal grains along with higher mean levels (4.3-161.0 ng/g) in positive samples than other toxins such as aflatoxins and ochratoxin A (less than 9% and below 5.2 ng/g in positive samples) from other fungal species.
建立了一种与传统方法相比有所改进的分析方法，用于通过液相色谱 - 串联质谱法（LC/MS/MS）在单次免疫亲和柱净化后同时测定谷物中的13种霉菌毒素（脱氧雪腐镰刀菌烯醇、雪腐镰刀菌烯醇、3 - 乙酰雪腐镰刀菌烯醇、黄曲霉毒素B₁、黄曲霉毒素B₂、黄曲霉毒素G₁、黄曲霉毒素G₂、伏马毒素B₁、伏马毒素B₂、T - 2毒素、HT - 2毒素、玉米赤霉烯酮和赭曲霉毒素A）。该方法在通过LC/MS/MS测定霉菌毒素时显示出良好的线性、灵敏度、特异性和准确性。对来自韩国的5种商业谷物（糙米、玉米、小米、高粱和混合谷物）中的13种霉菌毒素水平进行了测定，共检测了507份谷物样本。由 种产生的霉菌毒素（伏马毒素、脱氧雪腐镰刀菌烯醇、雪腐镰刀菌烯醇和玉米赤霉烯酮）在所有谷物中更频繁（超过5%）且同时被检测到，阳性样本中的平均水平（4.3 - 161.0 ng/g）高于其他真菌物种产生的其他毒素，如黄曲霉毒素和赭曲霉毒素A（阳性样本中低于9%且低于5.2 ng/g）。
