Fu Guanghua, Cheng Longfei, Fu Qiuling, Qi Baomin, Chen Cuiteng, Shi Shaohua, Chen Hongmei, Wan Chunhe, Liu Rongchang, Huang Yu
A Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, P. R. China.
B College of Animal Husbandry and Veterinary Medicine, Fujian Agriculture and Forestry University, Fuzhou 350002, P. R. China.
Avian Dis. 2017 Mar;61(1):33-39. doi: 10.1637/11440-053016-Reg.
Isolations of genotype IX (gIX) avian paramyxovirus type 1 (APMV-1) from various bird species have been more common recently, with isolates showing variable pathogenicity in different species of poultry. Here we sequenced the genome of a Muscovy duck origin gIX virus strain XBT14 and characterized the virulence and pathogenicity of this isolate in chickens and ducks. The genome sequence of strain XBT14 is 15,192 nt in length, containing multiple basic amino acids at the fusion protein cleavage site. The XBT14 strain shared 91.6%-91.9% nucleotide identities with early-genotype viruses (such as genotype III and IV) and shared 85.3%-85.9% nucleotide homologies with later genotype viruses (such as genotype VII). Pathogenicity tests showed that strain XBT14 could cause death in different duck breeds with a mortality rate of 44.4% in Muscovy duck, 25.9% in Sheldrake, and 11.1% in Cherry Valley duck, respectively. Similar mortality discrepancies were also observed in different ducks when infected with chicken-origin gIX virus strain F48E8. These results indicate that XBT14-like velogenic gIX APMV-1 (such as XBT14, F48E8, and GD09-2) could cause fatal infection in duck, and genotype IX is another genotype velogenic to duck as well as genotype VII. Accompanied by genetic differences in the vaccine strains or dominant strains prevailing in poultry, the virulent XBT14-like gIX viruses might become potentially endemic strains in poultry in the future.
最近，从各种鸟类中分离出基因型IX（gIX）的1型禽副粘病毒（APMV-1）的情况更为常见，分离株在不同家禽物种中表现出不同的致病性。在此，我们对一株番鸭源gIX病毒株XBT14的基因组进行了测序，并对该分离株在鸡和鸭中的毒力和致病性进行了表征。XBT14株的基因组序列长度为15,192 nt，在融合蛋白裂解位点含有多个碱性氨基酸。XBT14株与早期基因型病毒（如基因型III和IV）的核苷酸同一性为91.6%-91.9%，与后期基因型病毒（如基因型VII）的核苷酸同源性为85.3%-85.9%。致病性试验表明，XBT14株可导致不同鸭品种死亡，番鸭的死亡率为44.4%，麻鸭为25.9%，樱桃谷鸭为11.1%。当用鸡源gIX病毒株F48E8感染时，在不同鸭中也观察到类似的死亡率差异。这些结果表明，XBT14样强毒株gIX APMV-1（如XBT14、F48E8和GD09-2）可导致鸭的致命感染，基因型IX是另一种对鸭具有强致病性的基因型，与基因型VII一样。伴随着家禽中流行的疫苗株或优势株的基因差异，毒力强的XBT14样gIX病毒未来可能成为家禽中的潜在地方流行株。
