Choi Kang-Seuk, Kim Ji-Ye, Kye Soo-Jeong, Park Choi-Kyu, Sung Haan-Woo
Avian Disease Division, Animal, Plant and Fisheries Quarantine and Inspection Agency, Anyang, Gyeonggi 430-757, Republic of Korea.
Virus Genes. 2013 Apr;46(2):302-8. doi: 10.1007/s11262-012-0860-4. Epub 2012 Dec 13.
Thirteen isolates of avian paramyxovirus type 4 (APMV-4) isolated from wild ducks in Korea from 2006 to 2011 were genetically characterized by sequence analysis of the N-terminal region of the APMV-4 fusion (F) protein gene. The results revealed that the amino acid sequence homology within Korean isolates was 97.5 % or greater. The homologies of the Korean isolates with the APMV-4/duck/HK/D3/75 and APMV-4/duck/BE/15129/07 strains were 86.9-88.0 and 95.5-96.1 %, respectively. All Korean isolates had sequence motifs of (116)DIQPR↓F(121) at the F0 cleavage site. Phylogenetic analysis based on the N-terminal region of the F protein gene of APMV-4 isolates revealed that all 2006-2011 Korean isolates formed a single genotypic cluster that was phylogenetically different from APMV-4/duck/HK/D3/75 or APMV-4/duck/BE/15129/07 strains. Korean APMV-4 isolates were more closely related to APMV-4/goose/ZA/N1468/10 (isolated in South Africa) than to the Belgium APMV-4 virus. Korean APMV-4 isolates were further divided into at least two subgroups (A and B) based on phylogenetic analysis. Subgroup A viruses were isolated throughout Korea, whereas subgroup B viruses were detected only in isolates from Cheju island in 2011, suggesting that Korean APMV-4 exhibits marked genetic diversity and differs from viruses currently circulating in Europe and other locations.
对2006年至2011年从韩国野鸭中分离出的13株禽副粘病毒4型（APMV-4），通过对APMV-4融合（F）蛋白基因N端区域进行序列分析来进行基因特征鉴定。结果显示，韩国分离株之间的氨基酸序列同源性为97.5%或更高。韩国分离株与APMV-4/鸭/香港/D3/75和APMV-4/鸭/比利时/15129/07毒株的同源性分别为86.9 - 88.0%和95.5 - 96.1%。所有韩国分离株在F0裂解位点具有(116)DIQPR↓F(121)的序列基序。基于APMV-4分离株F蛋白基因N端区域的系统发育分析表明，所有2006 - 2011年的韩国分离株形成了一个单一的基因型簇，在系统发育上与APMV-4/鸭/香港/D3/75或APMV-4/鸭/比利时/15129/0
