Choi Kang-Seuk, Kim Ji-Ye, Lee Hyun-Jeong, Jang Min-Jun, Kwon Hyuk-Moo, Sung Haan-Woo
1 World Organisation for Animal Health (OIE) Reference Laboratory for Newcastle Disease, Avian Disease Research Division, Animal and Plant Quarantine Agency, Gimcheon, Gyeongbuk 39660, Republic of Korea.
2 Animal Veterinary Drugs and Biologics Division, Animal and Plant Quarantine Agency, Gimcheon, Gyeongbuk 39660, Republic of Korea.
J Wildl Dis. 2018 Jul;54(3):558-563. doi: 10.7589/2017-07-158. Epub 2018 Mar 8.
Eleven avian paramyxovirus type 6 (APMV-6) isolates from Eurasian Wigeon ( n=5; Anas penelope), Mallards ( n=2; Anas platyrhynchos), and unknown species of wild ducks ( n=4) from Korea were analyzed based on the nucleotide (nt) and deduced amino acid sequences of the fusion (F) gene. Fecal samples were collected in 2010-14. Genotypes were assigned based on phylogenetic analyses. Our results revealed that APMV-6 could be classified into at least two distinct genotypes, G1 and G2. The open reading frame (ORF) of the G1 genotype was 1,668 nt in length, and the putative F0 cleavage site sequence was PAPEPRL. The G2 genotype viruses included five isolates from Eurasian wigeons and four isolates from unknown waterfowl species, together with two reference APMV-6 strains from the Red-necked Stint ( Calidris ruficollis) from Japan and an unknown duck from Italy. There was an N-truncated ORF (1,638 nt), due to an N-terminal truncation of 30 nt in the signal peptide region of the F gene, and the putative F0 cleavage site sequence was SIREPRL. The genetic diversity and ecology of APMV-6 are discussed.
基于融合（F）基因的核苷酸（nt）和推导的氨基酸序列，对来自韩国的11株禽副粘病毒6型（APMV-6）分离株进行了分析，这些分离株分别来自欧亚野鸭（n=5；绿头鸭）、绿头鸭（n=2；绿头鸭）和未知种类的野鸭（n=4）。粪便样本于2010年至2014年采集。根据系统发育分析确定基因型。我们的结果表明，APMV-6可分为至少两个不同的基因型，即G1和G2。G1基因型的开放阅读框（ORF）长度为1668 nt，推测的F0裂解位点序列为PAPEPRL。G2基因型病毒包括来自欧亚野鸭的5株分离株、来自未知水禽种类的4株分离株，以及来自日本红颈滨鹬（红颈滨鹬）和意大利一只未知鸭子的两株APMV-6参考毒株。由于F基因信号肽区域N端截短30 nt，存在一个N端截短的ORF（1638 nt），推测的F0裂解位点序列为SIREPRL。本文讨论了APMV-6的遗传多样性和生态学。
