Jiang Huanhuan, Ma Xiang, Zhou Wenjuan, Dong Kai, Rausch-Fan Xiaohui, Liu Shutai, Li Shu
*PhD Student, Department of Periodontology, School of Stomatology, Shandong University, Jinan, China; Resident Physician, Department of Dental Implantology, Yantai Stomatological Hospital, Yantai, China. †MSc Student, Department of Stomatology, Binzhou Medical University, Yantai, China. ‡PhD Student, Department of Stomatology, Medical University of Vienna, Vienna, Austria. §Resident Physician, Department of Dental Implantology, Yantai Stomatological Hospital, Yantai, China. ¶Professor, Orthodontics & Periodontology Research Unit, Bernhard-Gottlieb-University Clinic of Dentistry, Sensengasse, Vienna, Austria. ‖Associate Chief Physician, Department of Dental Implantology, Yantai Stomatological Hospital, Yantai, China. #Professor, Department of Periodontology, School of Stomatology, Shandong University, Jinan, China.
Implant Dent. 2017 Apr;26(2):263-269. doi: 10.1097/ID.0000000000000576.
The aim of the present study was to mimic the hierarchical structure of bone tissues by simple sandblasting/acid-etching and anodization to investigate the effects of such surface characteristics on proliferation and differentiation of osteoblasts in high glucose concentrations. By the way, the effects of high glucose levels on osteoblast functions were tested.
MC3T3-E1 cells cultured on sand-blasted and acid-etched (SLA) surface and nano-modified SLA (NMSLA) surface were subjected to normal serum (NS) and diabetic serum (DS), respectively. The surface characteristics were evaluated by scanning electron microscopy. Cell proliferation was assessed using MTT assay. The levels of alkaline phosphatase (ALP) activity and mineralization were measured and compared. Real-time polymerase chain reaction was applied to detect the expression levels of osteogenic genes.
NMSLA significantly increased cell proliferation at time points ranging from 3 to 7 days under both serums. Cells cultured on NMSLA surfaces displayed significantly higher ALP activities and mineralization. The expression levels of Runx2 (indicates runt-related protein 2), collagen I (COL1), and osteocalcin (OCN) were notably increased on NMSLA surface compared with SLA surface. Moreover, we found that high glucose increased osteoblast proliferation but decreased differentiation of osteoblast slightly.
The hierarchical micro/nano-structured titanium surface has a favorable biocompatibility on simultaneously improving osteoblast proliferation and differentiation in diabetic serum.
本研究旨在通过简单的喷砂/酸蚀和阳极氧化模拟骨组织的层次结构，以研究这种表面特性对高糖浓度下成骨细胞增殖和分化的影响。顺便测试了高糖水平对成骨细胞功能的影响。
将培养在喷砂和酸蚀（SLA）表面及纳米改性SLA（NMSLA）表面的MC3T3-E1细胞分别置于正常血清（NS）和糖尿病血清（DS）中。通过扫描电子显微镜评估表面特性。使用MTT法评估细胞增殖。测量并比较碱性磷酸酶（ALP）活性和矿化水平。应用实时聚合酶链反应检测成骨基因的表达水平。
在两种血清下，NMSLA在3至7天的时间点显著增加细胞增殖。在NMSLA表面培养的细胞显示出显著更高的ALP活性和矿化。与SLA表面相比，NMSLA表面上Runx2（表明与矮小相关蛋白2）、I型胶原（COL1）和骨钙素（OCN）的表达水平显著增加。此外，我们发现高糖增加了成骨细胞增殖，但略微降低了成骨细胞的分化。
分级微/纳米结构钛表面在改善糖尿病血清中成骨细胞增殖和分化方面具有良好的生物相容性。
