Júnior José Augusto Gabarra, Nóbrega Fernando, Oliveira Paula Gabriela, Bergamo Edmara Tatiely, Cadore Uislen, Gomes Milene Zezzi do Valle, Kjellin Per, Chaushu Liat, Bezerra Fabio, Ghiraldini Bruna, Scombatti de Souza Sergio
Department of Oral and Maxillofacial Surgery and Periodontology, School of Dentistry of Ribeirão Preto, University of Sao Paulo, Ribeirão Preto 14040-904, SP, Brazil.
Department of Periodontology, School of Dentistry, University Center of State of Para, Belem 66060-575, PA, Brazil.
J Funct Biomater. 2023 Jul 14;14(7):370. doi: 10.3390/jfb14070370.
Leukocyte-platelet-rich fibrin (L-PRF) contains growth factors that stimulate bone regeneration. This study evaluated the bone repair in a tibia rat model around two implant surfaces in combination or not with L-PRF by assessing microtomographic and histomorphometric parameters. A total of 48 female rats were used in the study, in which 24 received implants with two types of surface treatments (dual acid etched-DAE or nanohydroxyapatite-nanoHA), and the other 24 received the same mini implants with L-PRF, which was collected by cardiac puncture, centrifugated, and inserted in the bone bed. The animals were euthanized 7 and 30 days after implant placement, and the retrieved samples were prepared for microtomographic and histomorphometric (bone-to-implant contact-BIC; and Bone Area Fraction Occupancy-BAFO) analyses. The adhesion of the nanoHA surface onto the implant surface was investigated by insertion and removal in simulated bone medium (Sawbones). The adhesion evaluation revealed that the loss of nanoHA after this procedure (as measured with SEM) from the implant surface was less than 1%. Overall, the nanoHA surface presented more bone in contact and in proximity to the implant, a higher bone surface/tissue volume fraction, a higher number of bone trabeculae, as well as trabecular separation relative to the DAE surface. Such results were more evident when the nanoHA surface was combined with L-PRF and after 30 days in vivo. The nanoHA surface presented higher BAFO when compared to DAE, with or without association with L-PRF. Therefore, implants with a nanoHA surface potentially benefit from the association to L-PRF.
富含白细胞-血小板纤维蛋白（L-PRF）含有刺激骨再生的生长因子。本研究通过评估显微断层扫描和组织形态计量学参数，在大鼠胫骨模型中评估了两种种植体表面单独或联合L-PRF后的骨修复情况。本研究共使用了48只雌性大鼠，其中24只接受了两种表面处理类型（双酸蚀刻-DAE或纳米羟基磷灰石-nanoHA）的种植体，另外24只接受了同样的微型种植体并联合L-PRF，L-PRF通过心脏穿刺采集、离心后植入骨床。在种植体植入后7天和30天对动物实施安乐死，并对取出的样本进行显微断层扫描和组织形态计量学分析（骨与种植体接触率-BIC；以及骨面积分数占有率-BAFO）。通过在模拟骨介质（Sawbones）中插入和取出，研究了nanoHA表面在种植体表面的附着力。附着力评估显示，在此过程后（用扫描电子显微镜测量），种植体表面nanoHA的损失小于1%。总体而言，与DAE表面相比，nanoHA表面与种植体接触和邻近的骨更多，骨表面积/组织体积分数更高，骨小梁数量更多，小梁间距更小。当nanoHA表面与L-PRF联合且在体内植入30天后，这些结果更为明显。与DAE相比，无论是否联合L-PRF，nanoHA表面的BAFO都更高。因此，具有nanoHA表面的种植体可能受益于与L-PRF的联合使用。
