Garcia R, Gauquelin G, Cantin M, Schiffrin E L
Laboratory of Experimental Hypertension and Vasoactive Peptides, Clinical Research Institute of Montreal, Quebec, Canada.
Hypertension. 1988 Feb;11(2):185-90. doi: 10.1161/01.hyp.11.2.185.
One-kidney, one clip (1K1C) hypertension is often associated with an expanded plasma volume and (once the arterial clip is removed) with natriuresis. Blood pressure (BP), atrial and plasma concentrations of atrial natriuretic factor (ANF), hematocrit, and renal glomerular ANF receptors were therefore studied in 1K1C rats and in their normotensive uninephrectomized controls before and after unclipping. Six hours after removal of the clip, BP was normal in the 1K1C group and plasma ANF presented a sharp decline but was still significantly higher than in the normotensive controls, with a slight difference being evident 24 hours after unclipping. Hematocrit was lower in the 1K1C rats than in their control counterparts, but this difference tended to disappear once the clip was removed, indicating a contraction of plasma volume in these unclipped 1K1C animals. The renal glomerular ANF receptor population was markedly smaller in 1K1C rats than in the uninephrectomized controls but showed a twofold increase in number and affinity 24 hours after unclipping. It is concluded that the up-regulation and enhanced affinity of glomerular ANF receptors (probably secondary to the decrease in plasma levels of ANF) may contribute to the natriuresis reported in hypertensive 1K1C animals on removal of the arterial clip.
单肾单夹（1K1C）高血压常伴有血浆容量扩张，并且（一旦移除动脉夹）伴有利钠作用。因此，在夹闭和解除夹闭前后，对1K1C大鼠及其血压正常的单肾切除对照大鼠的血压（BP）、心房和血浆中的心钠素（ANF）浓度、血细胞比容以及肾肾小球ANF受体进行了研究。解除夹闭6小时后，1K1C组的血压恢复正常，血浆ANF急剧下降，但仍显著高于血压正常的对照组，解除夹闭24小时后差异略有明显。1K1C大鼠的血细胞比容低于其对照大鼠，但一旦移除夹闭，这种差异趋于消失，表明这些解除夹闭的1K1C动物的血浆容量收缩。1K1C大鼠的肾肾小球ANF受体数量明显少于单肾切除对照大鼠，但在解除夹闭24小时后，其数量和亲和力增加了两倍。结论是，肾小球ANF受体的上调和亲和力增强（可能继发于ANF血浆水平的降低）可能有助于解释高血压1K1C动物在移除动脉夹后出现的利钠作用。
