Sivasankaran Unni, Jesny Sirijagan, Jose Ammu Rosin, Girish Kumar Krishnapillai
Department of Applied Chemistry, Cochin University of Science and Technology.
Anal Sci. 2017;33(3):281-285. doi: 10.2116/analsci.33.281.
Glutathione is an important antioxidant found in body fluids and tissues, which inhibit damage to essential cellular constituents caused by reactive oxygen species. The analysis of glutathione levels in biological systems is important in early clinical diagnosis. A novel, cost-effective synthetic strategy has been developed for the fluorescent probe ethylenediamine passivated carbon dots. Tissue paper was chosen as the carbon source for this "green one pot" synthesis. Glutathione could induce quenching of the fluorescence intensity of ethylenediamine passivated carbon dots through surface interactions, resulting from their aggregation. Based on this, a novel fluorescence sensor was fabricated for the determination of Glutathione in body fluids. A linear calibration graph was obtained in the range of 6.0 × 10 to 5.0 × 10 M with a detection limit of 1.74 × 10 M. The developed sensor was successfully used for the determination of glutathione in artificial saliva samples.
谷胱甘肽是一种存在于体液和组织中的重要抗氧化剂，它能抑制活性氧对细胞重要成分的损伤。生物系统中谷胱甘肽水平的分析在早期临床诊断中很重要。针对荧光探针乙二胺钝化碳点，已开发出一种新颖且经济高效的合成策略。选用薄页纸作为这种“绿色一锅法”合成的碳源。谷胱甘肽可通过表面相互作用诱导乙二胺钝化碳点的荧光强度猝灭，这是由它们的聚集导致的。基于此，制备了一种新型荧光传感器用于测定体液中的谷胱甘肽。在6.0×10至5.0×10 M范围内获得了线性校准曲线，检测限为1.74×10 M。所开发的传感器已成功用于测定人工唾液样品中的谷胱甘肽。
