Skolnik P R, Kosloff B R, Hirsch M S
Department of Medicine, Massachusetts General Hospital, Boston 02114.
J Infect Dis. 1988 Mar;157(3):508-14. doi: 10.1093/infdis/157.3.508.
Interactions between human immunodeficiency virus type 1 (HIV-1), the etiologic agent of AIDS, and human cytomegalovirus (CMV), a frequent opportunistic agent in AIDS, were studied in vitro. Coinfection of H9 cells with HIV-1 enhances productive CMV infection, as measured by immunofluorescence using monoclonal antibodies to late CMV proteins, slot-blot hybridization for CMV DNA, and cytopathic effects of CMV on human embryonic lung cells. Experiments using vaccinia virus recombinants and Jurkat cells transfected with the transactivating (tat) gene of HIV-1 suggest that this enhancement is not mediated primarily by the tat protein. In addition, coinfection of H9 cells or a monocyte cell line with CMV and HIV-1 results in enhanced HIV-1 replication, as measured in a virus-yield assay or by radioimmunoassay for the p24 antigen of HIV-1. The interactions between HIV-1 and CMV are thus bidirectional.
在体外研究了1型人类免疫缺陷病毒（HIV-1，艾滋病的病原体）与人类巨细胞病毒（CMV，艾滋病中常见的机会性病原体）之间的相互作用。用HIV-1共感染H9细胞可增强CMV的有效感染，这可通过使用针对CMV晚期蛋白的单克隆抗体进行免疫荧光检测、CMV DNA的斑点杂交以及CMV对人胚肺细胞的细胞病变效应来衡量。使用痘苗病毒重组体和用HIV-1反式激活（tat）基因转染的Jurkat细胞进行的实验表明，这种增强作用主要不是由tat蛋白介导的。此外，用CMV和HIV-1共感染H9细胞或单核细胞系会导致HIV-1复制增强，这可通过病毒产量测定或针对HIV-1 p24抗原的放射免疫测定来衡量。因此，HIV-1与CMV之间的相互作用是双向的。
