Tiedemann Heinz, Tiedemann Hildegard, Born Jochen, Kocher-Becker U
Institut für Physiologische Chemie der Freien Universität Berlin, Deutschland.
Heiligenberg-Institut, Heiligenberg, Baden.
Wilhelm Roux Arch Entwickl Mech Org. 1969 Dec;163(4):316-324. doi: 10.1007/BF00577018.
Crude extracts from 9 days old chicken embryos containing neuralizing and mesodermalizing inducing factors as well as purified mesodermalizing factor were incubated with thioglycolic acid and with 2-Mercaptoethanol. The fractions were tested by implanting into early gastrulae ofTriturus orAmbystoma. The mesodermalizing factor is inactivated whereas the neuralizing factor does not lose its activity.
将含有神经诱导因子和中胚层诱导因子的9日龄鸡胚粗提物以及纯化的中胚层诱导因子与巯基乙酸和2-巯基乙醇一起孵育。通过植入蝾螈或美西螈的早期原肠胚来测试这些组分。中胚层诱导因子失活,而神经诱导因子不失活。