Gutzeit Herwig O
Institute for Biology I (Zoology) of the Albert-Ludwigs-University Freiburg, Albertstr. 21a, D-7800, Freiburg i.Br., Germany.
Wilehm Roux Arch Dev Biol. 1980 Jun;188(2):153-156. doi: 10.1007/BF00848807.
The synthesis of a protein which has been detected in blastoderm cells but not in pole cells (Gutzeit and Gehring 1979) has been studied further by means of two-dimensional gel electrophoresis. This protein could not be detected at the nuclear multiplication stage. The protein is translated from mRNA which is transcribed at the blastoderm stage since it is not synthesized in detectable amounts when embryos are injected with α-amanitin prior to the blastoderm stage. Also the protein could not be detected when RNA from freshly laid eggs was translated in vitro. Embryos from females which are homozygous for the mutationmat (3) 1 form pole cells but no blastoderm cells (Rice and Garen 1975). Thesemat (3) 1 embryos, as we will call them in this report, express the protein if aged for a period of time sufficient for completion of blastoderm cell formation in control wild-type embryos.mat (3) 1 embryos and embryos injected with α-amanitin show the same syndrome of visible developmental anomalies; however, the studied protein could only be detected inmat (3) 1 embryos but not in α-amanitin injected embryos.
已通过二维凝胶电泳对一种在囊胚层细胞中被检测到但在极细胞中未被检测到的蛋白质（古策特和盖林，1979年）的合成进行了进一步研究。在核增殖阶段未检测到这种蛋白质。该蛋白质由在囊胚层阶段转录的mRNA翻译而来，因为在囊胚层阶段之前向胚胎注射α-鹅膏蕈碱时，它不会以可检测到的量合成。此外，当体外翻译刚产下的卵的RNA时，也检测不到这种蛋白质。对于突变体mat(3)1纯合的雌性胚胎会形成极细胞，但不会形成囊胚层细胞（赖斯和加伦，1975年）。在本报告中我们将这些mat(3)1胚胎培养一段时间，使其达到对照野生型胚胎完成囊胚层细胞形成所需的时间，此时它们会表达这种蛋白质。mat(3)1胚胎和注射了α-鹅膏蕈碱的胚胎表现出相同的明显发育异常综合征；然而，所研究的蛋白质仅能在mat(3)1胚胎中检测到，而在注射了α-鹅膏蕈碱的胚胎中检测不到。
