肌球蛋白多肽的信使核糖核酸：从单个肌源性细胞培养物中分离
Messenger RNA for myosin polypeptides: isolation from single myogenic cell cultures.
作者信息
Strohman R C, Moss P S, Micou-Eastwood J, Spector D, Przybyla A, Paterson B
出版信息
Cell. 1977 Feb;10(2):265-73. doi: 10.1016/0092-8674(77)90220-3.
Messenger RNA which stimilates the synthesis of myosin heavy chain in a reticulocyte lysate has been isolated from single myogenic cell cultures. Specific myosin polypeptides have been identified by immunoprecipitation with an antibody made to purified adult chicken skeletal muscle myosin. This mRNA binds to oligo(dT)-cellulose, and an active fraction from sucrose gradients migrates as 26S on formamide-polyacrylamide gels. The relative amount of this RNA increases dramatically at the time of terminal differentiation.
已从单个肌原细胞培养物中分离出在网织红细胞裂解物中刺激肌球蛋白重链合成的信使核糖核酸。通过用针对纯化的成年鸡骨骼肌肌球蛋白制备的抗体进行免疫沉淀，已鉴定出特定的肌球蛋白多肽。这种信使核糖核酸与寡聚（dT）纤维素结合，蔗糖梯度中的活性部分在甲酰胺-聚丙烯酰胺凝胶上以26S迁移。在终末分化时，这种核糖核酸的相对量急剧增加。
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