Banerjee S P, Snyder S H, Cuatrecasas P, Greene L A
Proc Natl Acad Sci U S A. 1973 Sep;70(9):2519-23. doi: 10.1073/pnas.70.9.2519.
(125)I-Labeled nerve growth factor binds to membrane preparations from superior cervical ganglia of rabbit in a specific fashion indicative of an interaction with the physiological receptor. Of various tissues examined, binding was observed only in the superior cervical ganglia, a presumed target organ of nerve growth factor. Binding of labeled factor is displaced by nonradioactive factor but not by several other peptide hormones. Binding to ganglia of preparations of nerve growth factor treated with N-bromosuccinimide closely parallels their biological activity. Binding of nerve growth factor receptor is saturable with an affinity constant of about 0.2 nM, similar to the reported plasma levels of the factor. Nerve growth factor does not elicit insulin-like biological effects in isolated adipocytes, and it does not modify the binding of [(125)I]insulin or [(125)I]epidermal growth factor to fat cells or liver membranes.
（125）I标记的神经生长因子以一种特定的方式与兔颈上神经节的膜制剂结合，这表明它与生理受体相互作用。在检查的各种组织中，仅在颈上神经节中观察到结合，颈上神经节被认为是神经生长因子的靶器官。标记因子的结合可被非放射性因子取代，但不能被其他几种肽激素取代。用N-溴代琥珀酰亚胺处理的神经生长因子制剂与神经节的结合与其生物学活性密切平行。神经生长因子受体的结合具有饱和性，亲和常数约为0.2 nM，与报道的该因子血浆水平相似。神经生长因子在分离的脂肪细胞中不会引发胰岛素样生物学效应，并且它不会改变[（125）I]胰岛素或[（125）I]表皮生长因子与脂肪细胞或肝细胞膜的结合。
