Vanroelen Chris, Vakaet C A
Department of Anatomy and Embryology, State University Antwerp (R.U.C.A.), 171 Groenenborgerlaan, B-2020, Antwerpen, Belgium.
Wilehm Roux Arch Dev Biol. 1981 Jul;190(4):233-236. doi: 10.1007/BF00848309.
Chick blastoderms were cultured for 2 h in the presence ofS-sulphate. The distribution of the grains after light microscope autoradiography was compared in blastoderms during the elongation and during the shortening of the primitive streak. A uniform labeling was observed over the cells in both groups. Accumulation of grains was present in both groups at the ventral side of the upper layer, where transmission electron microscope studies have revealed a basal lamina. An additional accumulation of grains occurred over the cells and in the extracellular spaces of the head process and of the rostral part of blastoderms with shortening primitive streaks. This positivity could be correlated with the presence of ingressing and recently ingressed notochordal cells. Treatment of the sections with chondroitinase ABC and/or HNO before dipping in the nuclear emulsion demonstrated that at least chondroitin sulphate and N-sulphated heparan sulphate were present.
将鸡胚盘在含S-硫酸盐的条件下培养2小时。在原条伸长和缩短过程中，通过光学显微镜放射自显影比较胚盘中银粒的分布。两组胚盘细胞上均观察到均匀的标记。在上层腹侧，两组均有银粒聚集，透射电子显微镜研究显示此处有基膜。在原条缩短的胚盘头部突起和头侧部分的细胞及细胞外间隙中，还出现了额外的银粒聚集。这种阳性反应可能与内迁和最近内迁的脊索细胞的存在有关。在浸入核乳胶之前，用软骨素酶ABC和/或硝酸处理切片表明，至少存在硫酸软骨素和N-硫酸化硫酸乙酰肝素。
