Vanroelen C, Harrisson F, Andries L, Vakaet L
J Microsc. 1981 Apr;122(Pt 1):1-5. doi: 10.1111/j.1365-2818.1981.tb01236.x.
Light microscope autoradiographs were prepared from a 14C line source Two factors that affect resolution were studied: emulsion thickness and section thickness. The distribution around the line source was determined using the half-distance (HD) value to quantify the resolution. An increase in HD value was observed with thicker sections or emulsion layers. The shape of the curve reflecting the grain density distributions around these line sources was very similar. After normalization in HD and relative grain density units, an average distribution was calculated and compared with the shape of normalized density distributions obtained from electron microscope autoradiographs. Other than a discrepancy near the source, an acceptable correlation was observed.
利用14C线状源制备了光学显微镜放射自显影片。研究了影响分辨率的两个因素：乳剂厚度和切片厚度。使用半距离（HD）值来量化分辨率，从而确定线状源周围的分布情况。观察到随着切片或乳剂层变厚，HD值会增加。反映这些线状源周围颗粒密度分布的曲线形状非常相似。在将HD和相对颗粒密度单位归一化后，计算出平均分布，并与从电子显微镜放射自显影片获得的归一化密度分布形状进行比较。除了源附近存在差异外，观察到了可接受的相关性。
