Elinson Richard P, Paleček Jiří
Department of Zoology, University of Toronto, 25 Harbord Street, M5S 1Al, Toronto, Ontario, Canada.
Department of Physiology and Developmental Biology, Faculty of Science, Charles University, 12800 Vinicna 7, Prague 2, Czechoslovakia.
Rouxs Arch Dev Biol. 1993 Apr;202(4):224-232. doi: 10.1007/BF02427883.
Two microtubule-containing structures are implicated in dorsoventral polarization of the frog egg, and we examined the relationship between them. The sperm aster provides a directional cue for a cortical rotation specifying polarity, and a vegetal cortical array of parallel microtubules is likely part of the rotational machinery. The growing aster has an accumulation of microtubules marking the path of the sperm pronucleus, and its microtubules extend into the egg cortex as well as the cytoplasm. To test whether the vegetal parallel array was an extension of astral cortical growth, fertilized or activated eggs were bisected into animal and vegetal fragments. The vegetal fragments formed parallel arrays, even when isolated within a few minutes of egg activation. Neither the sperm centrosome nor another microtubule organizing center in the animal half of the egg is required for formation of the parallel array, but some animal half activity is involved in its disappearance.
两种含有微管的结构与蛙卵的背腹极化有关，我们研究了它们之间的关系。精子星体为确定极性的皮层旋转提供了方向线索，而平行微管的植物皮层阵列可能是旋转机制的一部分。生长中的星体有微管聚集，标记着精子原核的路径，其微管延伸到卵皮层以及细胞质中。为了测试植物平行阵列是否是星体皮层生长的延伸，将受精或激活的卵切成动物和植物碎片。即使在卵激活后几分钟内分离，植物碎片也会形成平行阵列。平行阵列的形成不需要精子中心体或卵动物半侧的另一个微管组织中心，但动物半侧的一些活性参与了其消失过程。
