5-溴脱氧尿苷对牙胚的“体外”作用。II. - 对胶原蛋白合成的影响。

Action of 5-bromodeoxyuridine on tooth germs "in vitro". II. - Effects on collagen synthesis.

作者信息

Lesot H, Karcher-Djuricic V, Osman M, Ruch J V

出版信息

J Biol Buccale. 1978 Dec;6(4):281-91.

Abstract

The first lower molar tooth germs removed from 16-day-old mouse embryos were cultured for 2 days on a standard medium and then for 24 hours on the same medium containing BrdU (treated tooth germs) or not (controls). We attempted to study the effects of 5-Bromodeoxyuridine on the type I and type III collagen synthesis 24 (stage 16 + 4) and 72 (stage 16 + 6) hours after the incorporation of this thymidine analogue. At stage 16 + 4, type I and type III collagen were synthesized both in control and BrdU-treated tooth germs. However BrdU induced quantitative modifications in the type I collagen synthesis which might be explained by modifications in the turnover of this type of collagen. At stage 16 + 6, the BrdU treatment resulted in the inhibition of the terminal differentiation of odontoblasts. Consequently, the normal amplification of collagen type I synthesis could not occur. However, both control and BrdU-treated tooth germs synthesized type I and type III collagen. Quantitatively, the synthesis of type III collagen was slightly affected by BrdU treatment.

摘要

从16日龄小鼠胚胎中取出的第一颗下颌磨牙牙胚在标准培养基上培养2天，然后在含有溴脱氧尿苷（处理过的牙胚）或不含溴脱氧尿苷（对照）的相同培养基上培养24小时。我们试图研究在掺入这种胸腺嘧啶类似物后24小时（第16 + 4阶段）和72小时（第16 + 6阶段）5-溴脱氧尿苷对I型和III型胶原蛋白合成的影响。在第16 + 4阶段，对照牙胚和经溴脱氧尿苷处理的牙胚均合成I型和III型胶原蛋白。然而，溴脱氧尿苷诱导了I型胶原蛋白合成的定量改变，这可能是由这种类型胶原蛋白周转的改变所解释的。在第16 + 6阶段，溴脱氧尿苷处理导致成牙本质细胞的终末分化受到抑制。因此，I型胶原蛋白合成的正常扩增无法发生。然而，对照牙胚和经溴脱氧尿苷处理的牙胚均合成I型和III型胶原蛋白。从数量上看，III型胶原蛋白的合成受到溴脱氧尿苷处理的轻微影响。