Nagata K, Ichikawa Y
J Cell Physiol. 1979 Jan;98(1):167-75. doi: 10.1002/jcp.1040980118.
In a myeloid leukemia cell line, the inducibilities of the Fc receptor, phagocytosis and cell motility were compared. Thymidine analogues such as BUdR, BCdR and IUdR blocked the induction of phagocytosis and motility but not induction of the Fc receptor. This BUdR susceptibility in the induction of phagocytosis and motility was lost in a BUdR resistant line which was isolated for its growth capability in a high concentration of BUdR. Actinomycin D and puromycin brought about a marked decrease in the inducibility of phagocytosis but not in that of the Fc receptor. This led us to the following conclusion: There is a genetic control in the inducibility of phagocytosis and motility in this cell line, and the incorporation of BUdR into cellular DNA results in the DNA becoming unresponsive to a differentiation-stimulating factor. In contrast, gene activation does not seem to be necessary for induction of the Fc receptor. The order of induction of several differentiation markers was also discussed.
在一种髓系白血病细胞系中，对Fc受体的诱导性、吞噬作用和细胞运动性进行了比较。胸苷类似物如溴脱氧尿苷（BUdR）、氯脱氧尿苷（BCdR）和碘脱氧尿苷（IUdR）可阻断吞噬作用和运动性的诱导，但不影响Fc受体的诱导。在因能在高浓度BUdR中生长而分离出的对BUdR耐药的细胞系中，吞噬作用和运动性诱导过程中对BUdR的敏感性丧失。放线菌素D和嘌呤霉素使吞噬作用的诱导性显著降低，但不影响Fc受体的诱导性。由此我们得出以下结论：该细胞系中吞噬作用和运动性的诱导存在遗传控制，BUdR掺入细胞DNA会导致DNA对分化刺激因子无反应。相比之下，Fc受体的诱导似乎不需要基因激活。还讨论了几种分化标志物的诱导顺序。
