Nakayasu M, Terada M, Tamura G, Sugimura T
Proc Natl Acad Sci U S A. 1980 Jan;77(1):409-13. doi: 10.1073/pnas.77.1.409.
Tunicamycin, an antibiotic that specifically blocks the synthesis of N-acetylglucosamine-lipid intermediates and thereby prevents glycosylation of glycoproteins, induced differentiation of both human (HL-60) and murine (M1) myeloid leukemia cell lines in culture. At 0.1-1.0 microgram/ml, it induced differentiation of both HL-60 and M1 cells, characterized by increase in phagocytic cells and changes to resemble mature myeloid cells. Fc receptors were also induced in M1 but not in HL-60 cells; induction of intracellular lysozyme activity was not detected in either HL-60 or M1 cells. With this concentration of tunicamycin, there was marked decrease in rate of incorporation of radioactive glucosamine into macromolecules and a decrease in the rate of DNA synthesis. These data show that glycosylation of cellular proteins has an important role in maintaining these myeloid leukemia cells in an undifferentiated state in culture. The results also indicate that induction of phagocytosis in both HL-60 and M1 myeloid leukemia cells and of Fc receptors in M1 cells does not require continued synthesis of the oligosaccharide portions of cellular proteins by the lipid-linked pathway.
衣霉素是一种抗生素，它能特异性地阻断N - 乙酰葡糖胺 - 脂质中间体的合成，从而阻止糖蛋白的糖基化，在培养中可诱导人（HL - 60）和小鼠（M1）髓系白血病细胞系分化。在0.1 - 1.0微克/毫升的浓度下，它可诱导HL - 60和M1细胞分化，其特征为吞噬细胞增多以及细胞形态转变为类似成熟髓系细胞。M1细胞可诱导产生Fc受体，但HL - 60细胞中未出现；在HL - 60和M1细胞中均未检测到细胞内溶菌酶活性的诱导。在这种衣霉素浓度下，放射性葡糖胺掺入大分子的速率显著降低，DNA合成速率也下降。这些数据表明，细胞蛋白质的糖基化在培养中维持这些髓系白血病细胞处于未分化状态方面具有重要作用。结果还表明，HL - 60和M1髓系白血病细胞中吞噬作用的诱导以及M1细胞中Fc受体的诱导并不需要通过脂质连接途径持续合成细胞蛋白质的寡糖部分。
