一种用于测定干物质消化率的简单酶分析方法及其在研究广食性食草动物食物选择方面的价值。
A simple enzyme assay for dry matter digestibility and its value in studying food selection by generalist herbivores.
作者信息
Choo Gillian M, Waterman Peter G, McKey Doyle B, Gartlan J Stephen
机构信息
Phytochemistry Research Laboratory, Department of Pharmaceutical Chemistry, University of Strathclyde, G1 1XW, Glasgow, Scotland, UK.
Primate Ecology Unit, Wisconsin Regional Primate Research Center, University of Wisconsin, 53706, Madison, WI, USA.
出版信息
Oecologia. 1981 May;49(2):170-178. doi: 10.1007/BF00349185.
The dry matter digestibility of 94 species of leaf was assayed by a simple method involving sequential treatment with pepsin and fungal cellulase enzymes. It was demonstrated that for foliage from rainforest trees of a wide range of dicotyledonous plant families the assay showed high positive correlation with estimates of dry matter digestibility obtained using rumenliquor from a fistulated steer. Both assays were found to reflect negative correlates of digestibility, notably fibre and condensed tannin, rather than the nutritional value of an item. The higher dry matter digestibility of immature leaves relative to mature leaves appeared to be accounted for by their lower fibre content. It is suggested that the pepsin/cellulase assay offers a cheap, quick, routine method of gaining information on the effects of some types of plant secondary compounds (digestibility reducers) on the 'food potential' of different kinds of foliage to herbivores. Its use in studies of herbivory in rainforest areas in relation to analyses for plant secondary compounds and food selection by herbivores is discussed.
采用一种简单的方法，即先用胃蛋白酶再用真菌纤维素酶依次处理，对94种树叶的干物质消化率进行了测定。结果表明，对于广泛的双子叶植物科雨林树木的叶子，该测定方法与使用来自瘘管牛的瘤胃液获得的干物质消化率估计值呈高度正相关。发现这两种测定方法都反映了消化率的负相关因素，特别是纤维和缩合单宁，而不是某一物品的营养价值。未成熟叶片相对于成熟叶片较高的干物质消化率似乎是由于其较低的纤维含量。有人认为，胃蛋白酶/纤维素酶测定法提供了一种廉价、快速的常规方法，可用于获取有关某些类型的植物次生化合物（消化率降低剂）对不同种类树叶对食草动物的“食物潜力”影响的信息。本文讨论了该方法在雨林地区食草动物研究中与植物次生化合物分析和食草动物食物选择相关的应用。
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