Müller-Taubenberger A, Westphal M, Jaeger E, Noegel A, Gerisch G
Max-Planck-Institut für Biochemie, Martinsried bei München, FRG.
FEBS Lett. 1988 Mar 14;229(2):273-8. doi: 10.1016/0014-5793(88)81139-6.
The complete sequence of a Dictyostelium discoideum cDNA is presented that codes for monoubiquitin extended at its C-terminus by a 52 amino acid tail. The sequence of both the ubiquitin portion and the tail is highly homologous to the one of Saccharomyces cerevisiae and to a partial mouse sequence. The highly basic tail sequence contains a putative metal and nucleic acid-binding motif. The gene encoding the 0.6 kb mRNA of the C-terminally extended ubiquitin is represented only once in the haploid genome. The 0.6 kb mRNA as well as its translation product, a 15 kDa protein, is expressed in exponentially growing cells and remains present for at least 5 h of development. Using antibodies against a synthetic peptide that corresponds to the C-terminal amino acid sequence, a 15 kDa protein containing the extension a synthetic peptide that corresponds to the C-terminal amino acid sequence, a 15 kDa protein containing the extension was also detected in yeast.
本文展示了一种盘基网柄菌（Dictyostelium discoideum）cDNA的完整序列，该序列编码在其C末端延伸有52个氨基酸尾巴的单泛素。泛素部分和尾巴的序列与酿酒酵母（Saccharomyces cerevisiae）的序列以及部分小鼠序列高度同源。高度碱性的尾巴序列包含一个推定的金属和核酸结合基序。编码C末端延伸泛素的0.6 kb mRNA的基因在单倍体基因组中仅出现一次。0.6 kb mRNA及其翻译产物，一种15 kDa的蛋白质，在指数生长的细胞中表达，并在发育至少5小时内持续存在。使用针对与C末端氨基酸序列对应的合成肽的抗体，在酵母中也检测到了一种含有该延伸序列的15 kDa蛋白质。
