人抗p68自身抗体识别包含小核核糖核蛋白的U1 RNA和乙型流感病毒的共同表位。
Human anti-p68 autoantibodies recognize a common epitope of U1 RNA containing small nuclear ribonucleoprotein and influenza B virus.
作者信息
Guldner H H, Netter H J, Szostecki C, Jaeger E, Will H
机构信息
Max-Planck-Institut für Biochemie, Martinsried, Federal Republic of Germany.
出版信息
J Exp Med. 1990 Mar 1;171(3):819-29. doi: 10.1084/jem.171.3.819.
Abstract
Autoantibodies from patients with systemic rheumatic diseases were used to map antigenic sites on the 68-kD autoantigen (p68) associated with (U1)RNA-containing small nuclear ribonucleoprotein (snRNP) particles. With truncated recombinant fusion proteins and synthetic peptides, a subset of anti-p68 autoantibodies was found to recognize the amino acid sequence motif Glu-Arg-Lys-Arg-Arg (ERKRR). To investigate the possible involvement of epitopes shared by microbial antigens and host self-components in initiation of autoimmunity (molecular mimicry), a sequence data bank was screened for proteins containing an amino acid motif identical or related to ERKRR. The identical motif was found on the M1 matrix protein of influenza B viruses, and affinity-purified human anti-ERKRR autoantibodies recognized this epitope also in the viral amino acid sequence context. The common epitope recognized by human autoantibodies suggests that influenza B virus infection may play a role in initiation of the anti-p68 and anti-(U1)RNP autoimmune response.
摘要
来自系统性风湿性疾病患者的自身抗体被用于绘制与含（U1）RNA的小核核糖核蛋白（snRNP）颗粒相关的68-kD自身抗原（p68）上的抗原位点。利用截短的重组融合蛋白和合成肽，发现一部分抗p68自身抗体可识别氨基酸序列基序Glu-Arg-Lys-Arg-Arg（ERKRR）。为了研究微生物抗原和宿主自身成分共有的表位在自身免疫起始过程中（分子模拟）的可能作用，在序列数据库中筛选含有与ERKRR相同或相关氨基酸基序的蛋白质。在乙型流感病毒的M1基质蛋白上发现了相同的基序，亲和纯化的人抗ERKRR自身抗体在病毒氨基酸序列背景下也能识别该表位。人自身抗体识别的共同表位提示乙型流感病毒感染可能在抗p68和抗（U1）RNP自身免疫反应的起始中起作用。
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