Kumar C Ganesh, Mongolla P, Pombala S, Bandi S, Babu K S, Ramakrishna K V S
Medicinal Chemistry and Pharmacology Division, CSIR-Indian Institute of Chemical Technology, Tarnaka, Hyderabad, India.
Department of Biotechnology, Acharya Nagarjuna University, Guntur, Andhra Pradesh, India.
J Appl Microbiol. 2017 Jun;122(6):1518-1528. doi: 10.1111/jam.13450. Epub 2017 May 8.
The aim of the study was to purify and characterize a bioactive compound from Aspergillus nidulans strain KZR-132 and its biological evaluation.
A bioactive extolite was purified from A. nidulans strain KZR-132, and its chemical structure was elucidated as 3-hydroxylbenzyl alcohol (3-HBA) based on H and C NMR, FT-IR and mass spectroscopic analysis. The antimicrobial efficacy of 3-HBA was established against Gram-positive, Gram-negative bacteria and different Candida strains. It also showed promising antibiofilm activity against various tested microbial strains. Reactive oxygen species induced by 3-HBA treatment on different Candida strains killed most of the cells and showed necrotic effect. It also exhibited dose-dependent antioxidant and anti-inflammatory activities.
This bioactive extrolite produced by A. nidulans isolated from a niche habitat was demonstrated to possess significant biotechnological and pharmacological potential since it exhibited broad-spectrum antimicrobial and antibiofilm activities which are reported for the first time.
The overall study demonstrates that 3-HBA produced by A. nidulansKZR-132 is a promising bioactive metabolite and possibly can function as a pharmacologically suitable broad-spectrum antimicrobial drug candidate against various dreaded human-related bacterial and fungal pathogens.
本研究旨在从构巢曲霉KZR - 132菌株中纯化并鉴定一种生物活性化合物，并对其进行生物学评价。
从构巢曲霉KZR - 132菌株中纯化出一种生物活性外泌物，基于氢谱、碳谱核磁共振、傅里叶变换红外光谱和质谱分析，其化学结构被鉴定为3 - 羟基苄醇（3 - HBA）。确定了3 - HBA对革兰氏阳性菌、革兰氏阴性菌及不同念珠菌菌株的抗菌效果。它对各种受试微生物菌株还表现出有前景的抗生物膜活性。3 - HBA处理不同念珠菌菌株诱导产生的活性氧杀死了大多数细胞，并显示出坏死效应。它还表现出剂量依赖性的抗氧化和抗炎活性。
从特定生态位栖息地分离得到的构巢曲霉产生的这种生物活性外泌物被证明具有显著的生物技术和药理学潜力，因为它表现出了首次报道的广谱抗菌和抗生物膜活性。
总体研究表明，构巢曲霉KZR - 132产生的3 - HBA是一种有前景的生物活性代谢物，可能可作为一种药理学上合适的广谱抗菌药物候选物，用于对抗各种可怕的与人类相关的细菌和真菌病原体。
