用超临界流体从温州蜜柑果皮中提取川陈皮素及其CRE介导的转录活性。
Extraction of nobiletin from Citrus Unshiu peels by supercritical fluid and its CRE-mediated transcriptional activity.
作者信息
Oba Chisato, Ota Masaki, Nomura Koichiro, Fujiwara Hironori, Takito Jiro, Sato Yoshiyuki, Ohizumi Yasushi, Inomata Hiroshi
机构信息
Research Center of Supercritical Fluid Technology, Tohoku University, 6-6-11-403, Aoba, Aramaki, Aoba-ku, Sendai, 980-8579, Japan.
Research Center of Supercritical Fluid Technology, Tohoku University, 6-6-11-403, Aoba, Aramaki, Aoba-ku, Sendai, 980-8579, Japan.
出版信息
Phytomedicine. 2017 Apr 15;27:33-38. doi: 10.1016/j.phymed.2017.01.014. Epub 2017 Jan 31.
BACKGROUND
Polymethoxyflavone (PMF) is one of bioactive compounds in Citrus Unshiu and included mainly in the peels rather than the fruits, seeds and leaves.
HYPOTHESIS/PURPOSE: Supercritical CO extraction is one candidate for selective extraction of polymethoxyflavone and in this study, supercritical CO extraction with/without ethanol entrainer from Citrus Unshiu peels was examined at a temperature of 333K and a pressure of 30MPa.
METHODS
CRE (cyclic AMP response element)-mediated transcriptional assay was examined by using the extracts from supercritical fluid extraction.
RESULTS
The results showed that extracts including nobiletin increased with increasing ethanol concentration in supercritical CO and the elapsed extraction time. Extracts at ethanol concentration of 5 mol% showed high CRE-mediated transcription activity. This can be caused by activity of the extract including nobiletin in addition to the other methoxylated flavonoid species such as tangeretin. Extracts at ethanol concentration of 50% showed the highest CRE-mediated transcription activity, which can be attributed to flavonoid glycoside such as hesperidin. From our investigations, flavonoid glycoside can be one of promoters of CRE-mediated transcription activity.
背景
多甲氧基黄酮（PMF）是温州蜜柑中的生物活性化合物之一，主要存在于果皮中，而非果实、种子和叶片中。
假设/目的：超临界CO₂萃取是选择性萃取多甲氧基黄酮的一种方法，在本研究中，于333K温度和30MPa压力下，对有无乙醇夹带剂的温州蜜柑皮超临界CO₂萃取进行了研究。
方法
通过超临界流体萃取提取物进行CRE（环磷酸腺苷反应元件）介导的转录分析。
结果
结果表明，超临界CO₂中，随着乙醇浓度增加和萃取时间延长，含川陈皮素的提取物增加。乙醇浓度为5mol%时的提取物显示出较高的CRE介导的转录活性。这可能是由于除了其他甲氧基化黄酮类化合物（如橘红素）外，含川陈皮素的提取物具有活性。乙醇浓度为50%时的提取物显示出最高的CRE介导的转录活性，这可归因于类黄酮糖苷（如橙皮苷）。根据我们的研究，类黄酮糖苷可能是CRE介导的转录活性的促进剂之一。
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