Dong Guoyi, Shang Zhouchun, Liu Longqi, Liu Chuanyu, Ge Yuping, Wang Quanlei, Wu Liang, Chen Fang, Li Baolin, Liu Xin, Xu Xun, Yang Huanming, Du Yutao, Jiang Hui
BGI-Shenzhen, Shenzhen, China.
Department of Regenerative Medicine, Tongji University School of Medicine, Shanghai 200092, China.
Biosci Rep. 2017 Apr 20;37(2). doi: 10.1042/BSR20170637. Print 2017 Apr 30.
Spermatogenic lineage has been directly generated in spermatogonial stem cell (SSC) conditions from human pluripotent stem cells (PSCs). However, it remains unknown whether mouse embryonic stem cells (ESCs) can directly differentiate into advanced male germ cell lineage in the same conditions. Here, we showed rather low efficiency of germ-like cell generation from mouse ESCs in SSC conditions. Interestingly, addition of retinoic acid (RA) into SSC conditions enabled efficient differentiation of mouse ESCs into germ-like cells, as shown by the activation of spermatogenesis-associated genes such as , , , , , , and In contrast, for cells cultured in control medium, the activation of the above genes barely occurred. In addition, RA with SSC conditions yielded colonies of Acrosin-expressing cells and the positive ratio reached a peak at day 6. Our work thus establishes a simple and cost-efficient approach for male germ like cell differentiation from mouse PSCs and may propose a useful strategy for studying spermatogenesis
在精子发生干细胞（SSC）条件下，已从人多能干细胞（PSC）直接生成了精子发生谱系。然而，尚不清楚小鼠胚胎干细胞（ESC）在相同条件下是否能直接分化为高级雄性生殖细胞谱系。在此，我们展示了在SSC条件下从小鼠ESC生成类生殖细胞的效率相当低。有趣的是，在SSC条件中添加视黄酸（RA）能使小鼠ESC高效分化为类生殖细胞，这表现为精子发生相关基因如 、 、 、 、 、 和 的激活。相比之下，对于在对照培养基中培养的细胞，上述基因几乎未被激活。此外，RA与SSC条件产生了表达顶体蛋白酶的细胞集落，且阳性率在第6天达到峰值。因此，我们的工作建立了一种简单且经济高效的从小鼠PSC分化生成雄性类生殖细胞的方法，并可能为研究精子发生提出一种有用的策略
