Kravtsov E G, Yermolayev A V, Anokhina I V, Yashina N V, Chesnokova V L, Dalin M V
Department of Microbiology, Russian University of Peoples' Friendship, Moscow
Bull Exp Biol Med. 2008 Feb;145(2):232-4. doi: 10.1007/s10517-008-0058-x.
A sampling of lactobacilli from the German National Collection of Microorganisms and L. fermentum 90 TS-4 (21) reference strain clone 3 (Russian Federation) were studied. The results indicate that the receptors on the surface of lactobacillus strains from the German collection had no structures complementary to type 1 fimbriae, though adhesins of some of them reacted with mannose and galactose receptors. Adhesion on a monolayer of continuous cell cultures showed that adhesion activity of lactobacilli was a function of many derivatives, and hence, the choice of a model for evaluation of the adhesion characteristics of the strain should be based on adhesins exhibiting universal properties in different test systems. One of them can be lectin-binding adhesin; its expression on the surface of cultures of lactobacilli from the German collection varies within the same range as was shown previously for lactobacilli, studied by the same criterion. The molecular weight of lectin-binding adhesin is 25-30 kDa, and the corresponding receptors are frequently present on various eukaryotic cells, and hence, cell models can be considered as the most adequate for studies of the competitive interactions between lactobacilli and adhesins of pathogenic microorganisms.
对来自德国国家微生物保藏中心的乳酸菌样本以及发酵乳杆菌90 TS - 4（21）参考菌株克隆3（俄罗斯联邦）进行了研究。结果表明，来自德国保藏中心的乳酸菌菌株表面受体没有与1型菌毛互补的结构，尽管其中一些黏附素能与甘露糖和半乳糖受体发生反应。在连续细胞培养单层上的黏附实验表明，乳酸菌的黏附活性是多种因素的函数，因此，选择评估菌株黏附特性的模型应基于在不同测试系统中表现出通用特性的黏附素。其中之一可以是凝集素结合黏附素；其在来自德国保藏中心的乳酸菌培养物表面的表达变化范围与之前以相同标准研究的乳酸菌相同。凝集素结合黏附素的分子量为25 - 30 kDa，相应的受体经常存在于各种真核细胞上，因此，细胞模型可被认为是研究乳酸菌与致病微生物黏附素之间竞争相互作用的最合适模型。
