Pan Yunbao, Zhou Fuling, He Chenyan, Hui Lingyun, Huang Tianhe, Wei Yongchang
Department of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, China.
Department of Hematology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, China.
Biomed Res Int. 2017;2017:6482842. doi: 10.1155/2017/6482842. Epub 2017 Feb 20.
Depression is the most common psychiatric disorder among cancer patients. Studies have not only highlighted that leptin and its receptor (LepRb) are independent poor prognostic factors in gastric cancer (GC) patients but also shown that the leptin-LepRb is necessary for antidepressant-like behaviors. In this study, we examined the serum and tissue leptin-LepRb expression in GC patients. Enzyme-linked immunosorbent assay showed that depressive GC patients had significantly higher serum leptin-LepRb than healthy donors. Leptin-LepRb levels in GC tissues were also significantly higher than in matched paracarcinoma tissues using real-time RT-PCR. Moreover, we observed that both serum and tissue leptin-LepRb were significantly higher in depressive GC patients than those in nondepressive GC patients. Further, the patients with high tumor stage tend to have higher leptin-LepRb mRNA levels than that with low tumor stage. Together, our findings suggest that leptin-LepRb plays an important role in the pathogenesis and depression in GC. Leptin-LepRb therefore could be a potential diagnostic marker and therapeutic target in GC patients with depression.
抑郁症是癌症患者中最常见的精神疾病。研究不仅强调瘦素及其受体（LepRb）是胃癌（GC）患者独立的不良预后因素，还表明瘦素-LepRb对抗抑郁样行为是必需的。在本研究中，我们检测了GC患者血清和组织中瘦素-LepRb的表达。酶联免疫吸附测定显示，抑郁的GC患者血清瘦素-LepRb显著高于健康供者。使用实时逆转录聚合酶链反应（RT-PCR）检测发现，GC组织中瘦素-LepRb水平也显著高于配对的癌旁组织。此外，我们观察到抑郁的GC患者血清和组织中的瘦素-LepRb均显著高于非抑郁的GC患者。此外，肿瘤分期高的患者瘦素-LepRb mRNA水平往往高于肿瘤分期低的患者。总之，我们的研究结果表明，瘦素-LepRb在GC的发病机制和抑郁中起重要作用。因此，瘦素-LepRb可能是GC伴抑郁患者的潜在诊断标志物和治疗靶点。
