Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran.
Poultry diseases resident, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran.
Biol Trace Elem Res. 2017 Nov;180(1):164-170. doi: 10.1007/s12011-017-0993-3. Epub 2017 Mar 20.
Pulmonary arterial hypertension (PAH) syndrome in broilers is associated with hypoxia, which prevails at high altitude. Oxidative stress is the pathogenic mechanism underlying PAH. Because selenium is key element in the structure of antioxidant enzymes, we evaluated pulmonary hypertensive responses in broiler chickens fed with diets supplemented with organic or nano-selenium. One hundred forty-four broilers (starting at 5 days old) were fed with (i) control group: birds received a standard diet; (ii) nano-selenium group: birds were fed with basal diet supplemented with nano-selenium at 0.3 mg/kg; and (iii) organic selenium group: birds received basal diet supplemented with organic selenium at 0.3 mg/kg. We assessed growth performance, carcass characteristics, antioxidant variables, blood parameters, and small intestine morphology. Although Se supplementation did not affect growth performance, carcass traits, and organ weight (P > 0.05), the right to total ventricular weight ratio (RV:TV), malondialdehyde concentration in the liver, and heterophil to lymphocyte ratio were significantly lower in the nano-selenium group relative to the control (P < 0.05). Chickens that received nano-selenium also elicited significantly higher antibody titers after 24 h of an injection of sheep red blood cells (P < 0.05). Nano-selenium supplementation also significantly increased villus height, absorptive surface area, and lamina propria thickness relative to the control (P < 0.05) in different segments of the small intestine. In contrast, organic selenium supplement improved intestinal morphometry only in the jejunum. We conclude that dietary supplementation of 0.30 mg/kg nano-selenium could prevent right ventricular hypertrophy as reflected by reduced RV:TV, reduced levels of lipid peroxidation in the liver, and improved gut function.
肉鸡肺动脉高压（PAH）综合征与缺氧有关，而缺氧在高海拔地区更为常见。氧化应激是 PAH 的发病机制。由于硒是抗氧化酶结构中的关键元素，因此我们评估了补充有机或纳米硒的饮食对肉鸡肺动脉高压反应的影响。将 144 只肉鸡（从 5 天大开始）分为三组：（i）对照组：鸡只接受标准饮食；（ii）纳米硒组：鸡只喂食基础饮食，同时补充 0.3mg/kg 的纳米硒；（iii）有机硒组：鸡只喂食基础饮食，同时补充 0.3mg/kg 的有机硒。我们评估了生长性能、屠体特征、抗氧化变量、血液参数和小肠形态。尽管硒补充并没有影响生长性能、屠体特征和器官重量（P>0.05），但纳米硒组的右心室总重量比（RV:TV）、肝脏丙二醛浓度和异嗜性白细胞与淋巴细胞比与对照组相比显著降低（P<0.05）。纳米硒组鸡在注射绵羊红细胞 24 小时后也产生了更高的抗体滴度（P<0.05）。与对照组相比，纳米硒补充还显著增加了不同肠段的绒毛高度、吸收表面积和固有层厚度（P<0.05）。相比之下，有机硒补充仅在空肠中改善了肠道形态计量学。我们得出结论，饮食补充 0.30mg/kg 的纳米硒可以预防 RV:TV 降低、肝脏脂质过氧化水平降低和肠道功能改善所反映的右心室肥大。
