ICAR- Central Avian Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India.
ICAR- Central Avian Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India.
J Therm Biol. 2020 Feb;88:102515. doi: 10.1016/j.jtherbio.2020.102515. Epub 2020 Jan 10.
Present study was carried out with the objective of investigating the role of green synthesized nano Se (GNS) in growth performance, digestibility of minerals, immunity, stress alleviation, antioxidant status, and body Se content of broiler chicken raised under hot and humid environment with respect to market nano Se (MNS) and inorganic Se. The experimental design was 3 × 3 factorial, in which three levels (0.15, 0.20, and 0.25 ppm) and three sources (inorganic, green nano, and market nano) of Se resulted in nine treatments viz. IS-0.15, GNS-0.15, MNS-0.15, IZ-0.20, GNS-0.20, MNS-0.20, IS-0.25, GNS-0.25, and MNS-0.25 (IS: inorganic Se, GNS: green nano Se, MNS: market nano Se). A total of 432 broiler chicken were divided among nine treatments with six replicates of birds per treatment (8 birds/replicate). Results of present study revealed significantly better growth performance of birds supplemented with 0.25 ppm nano Se. The supplementation of 0.25 ppm nano Se improved the immune response and lymphoid organ development of birds. Significantly higher Se and nitrogen digestibility coefficients, serum antioxidant activity and decline of Heterophil: Lymphocyte ratio and expression of HSP70 gene were observed in birds supplemented with 0.25 ppm Se and nano source of Se compared to inorganic Se. Significantly higher Se concentration in liver and breast muscle and higher serum Se concentration were observed in birds fed 0.25 ppm nano Se. The liver Se concentration was much higher than that of breast muscle. However, the nano Se synthesized by green method in this study did not differ significantly from the chemically synthesized nano Se. It was concluded that 0.25 ppm Se and nano form of Se are superior to lower levels and inorganic form of Se, respectively, in improving the immunity, growth, antioxidant status, and in stress alleviation of broiler chicken. However, GNS is equally efficient as chemically synthesized MNS.
本研究旨在探讨在湿热环境下，与市场纳米硒（MNS）和无机硒相比，绿色合成纳米硒（GNS）对肉鸡生长性能、矿物质消化率、免疫力、应激缓解、抗氧化状态和体内硒含量的影响。实验设计采用 3×3 因子设计，硒的三个水平（0.15、0.20 和 0.25ppm）和三个来源（无机、绿色纳米和市场纳米）共产生 9 种处理，即 IS-0.15、GNS-0.15、MNS-0.15、IZ-0.20、GNS-0.20、MNS-0.20、IS-0.25、GNS-0.25 和 MNS-0.25（IS：无机硒、GNS：绿色纳米硒、MNS：市场纳米硒）。总共 432 只肉鸡被分为 9 个处理组，每个处理组有 6 个重复，每个重复有 8 只鸡。本研究结果表明，补充 0.25ppm 纳米硒的鸡生长性能明显更好。补充 0.25ppm 纳米硒可提高鸡的免疫反应和淋巴器官发育。与无机硒相比，补充 0.25ppm 纳米硒可显著提高硒和氮的消化率系数、血清抗氧化活性以及异嗜细胞：淋巴细胞比值和 HSP70 基因的表达。与无机硒相比，补充 0.25ppm 纳米硒的鸡肝脏和胸肌中的硒浓度以及血清中的硒浓度更高。鸡肝脏中的硒浓度明显高于胸肌中的硒浓度。然而，本研究中通过绿色方法合成的纳米硒与化学合成的纳米硒没有显著差异。研究结果表明，0.25ppm 硒和纳米硒形式优于低水平和无机硒形式，分别可提高肉鸡的免疫力、生长性能、抗氧化状态和缓解应激。然而，GNS 与化学合成的 MNS 同样有效。
