Lambert T, Gerbaud G, Courvalin P
Hôpital Saint-Michel, Paris, France.
Antimicrob Agents Chemother. 1988 Jan;32(1):15-9. doi: 10.1128/AAC.32.1.15.
Acinetobacter baumannii BM2580 resistant to kanamycin and structurally related antibiotics, including amikacin, was isolated from a clinical specimen. A phosphocellulose paper-binding assay and DNA annealing studies indicated that resistance to aminoglycosides in BM2580 was due to synthesis of a new type of 3'-aminoglycoside phosphotransferase. The gene conferring resistance to kanamycin-amikacin in this strain was carried by a 63-kilobase plasmid, pIP1841, self-transferable to A. baumannii, A. haemolyticus, and A. lwoffii but not to Escherichia coli. The aminoglycoside resistance gene of pIP1841 was cloned in E. coli, where it was expressed.
从一份临床标本中分离出了对卡那霉素及包括阿米卡星在内的结构相关抗生素耐药的鲍曼不动杆菌BM2580。磷酸纤维素纸结合试验和DNA退火研究表明，BM2580对氨基糖苷类抗生素的耐药性是由于一种新型3'-氨基糖苷磷酸转移酶的合成。赋予该菌株对卡那霉素-阿米卡星耐药性的基因由一个63千碱基的质粒pIP1841携带，该质粒可自行转移至鲍曼不动杆菌、溶血不动杆菌和洛菲不动杆菌，但不能转移至大肠杆菌。pIP1841的氨基糖苷类耐药基因在大肠杆菌中克隆并表达。
